Genetics News

1
Genetics News
Lost book
Mystery sequence project
Problem Day Friday
Unknown Day Monday
2
Where to get help before the exam
Wednesday, Sept 20 630 PM - 800
PM E-107 Sarah Muller Wednesday, Sept 20
700 PM - 900 PM UFA2107 Jennifer
Lee Thursday, Sept 21 400 PM - 500
PM S-215 Garrett Griffin Friday, Sept 22
300 PM - 500 PM S-215 Jeff Elhai Sunday,
Sept 24 800 PM - 1000 PM E-209 Karen
Lewis Monday, Sept 25 230 PM - 330
PM S-111 Sarah Muller Monday, Sept 25
515 PM - 615 PM S-111 Sarah Muller
See Who We Are page on web.Click on Special Help
Sessions.
3
Pathways Making Autos
4
Pathways Making Autos
5
Pathways Making Autos
6
Pathways Making Autos
7
Pathways Making Autos
8
Pathways Making Autos
9
Pathways Making Autos
substrate
10
Pathways Making Autos
substrateconsumed
Product
11
Pathways Making Pigment
Enzymes
Cell

chemical A(vermilion color)
12
Pathways Making Pigment
Enzyme X
Modify chemical A
13
Pathways Making Pigment
Enzyme X
chemical B(cinnabar color)
14
Pathways Making Pigment
Enzyme Y
Modify chemical B
15
Pathways Making Pigment
Enzyme Y
Wild-type pigment(red color)
16
Pathways Making Pigment
Accumulate wild-type pigment
17
When Pathways Go Wrong
X Y
Wild-type eye color
X Y-
Cinnabar eye color
X- Y
Vermilion eye color
18
When Pathways Go Wrong
Imaginal disk tissue
X- Y
Abdominal tissue
X Y-
19
When Pathways Go Wrong
Imaginal disk tissue
X- Y
Wild-type eye color
Abdominal tissue
X Y-
20
Pulse-Chase ExperimentDoes RNA move from nucleus
to cytoplasm?
Pre pulse
RNA
U
U
U
U
U
U
U
Uracil
U
U
U
U
U
U
U
21
Pulse-Chase ExperimentDoes RNA move from nucleus
to cytoplasm?
Pulse
RNA
U
U
U
U
U
U
U
U
Uracil
U
U
U
U
U
U
U
U
22
Pulse-Chase ExperimentDoes RNA move from nucleus
to cytoplasm?
Chase
RNA
U
U
U
U
U
U
U
Uracil
U
U
U
U
U
U
U
23
Comparison of artificial RNA polymer experiment
amino acid swap experiment
Artificial polymer Amino acid swap
Experimental need
Need ribosomes, tRNA, tRNA synthetases
Cell extract
Cell extract
Label each aa, one at a time
Need to focus on one amino acid
Label cysteine
Need artificial polymer
Use poly(U,G)
Use poly(U,G)
Isolate new protein
Precipitate, filter
Precipitate, filter
24
Comparison of artificial RNA polymer experiment
amino acid swap experiment
Artificial polymer Amino acid swap
Experimental need
Need ribosomes, tRNA, tRNA synthetases
Cell extract
Cell extract
Label each aa, one at a time
Need to focus on one amino acid
Label cysteine
Alter amino acid on tRNA
Convert cysteine to alanine
---
Need artificial polymer
Use poly(U,G)
Use poly(U,G)
Isolate new protein
Precipitate, filter
Precipitate, filter
25
Classes of RNA and Why they are
26
(No Transcript)