Normal Flora

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Normal Flora

• Microbiotica

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Microbiotica
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Synder Deep

• CH2O fermentation
• Glucose
• Differential
• Rate of change
• susceptibility
• Selective for lactobacillus
• pH 4.8
• Indicator bromcresol green
• Dental caries

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BAP

• Divide Plate
• Swab throat
• Differential
• Sheep Blood
• Hemolysis
• Hemolysin exotoxins
• Types
• Gamma
• Alpha
• Beta

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Chocolate Agar

• Divide Plate
• Swab throat
• Differential
• Growth
• Oxidase test
• Neisseria
• Lysed RBCs

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MSA

• Selective
• Differential
• Divide plate
• Swab Skin
• Nose
• Face
• Results
• Fermentation
• acid end products
• yellow
• Components
• 7.5 NaCl
• mannitol
• pH indicator phenol red

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MH-T

• Divide plate
• Swab skin
• Ears
• Palms
• Differential
• Diptheroids
• Tellurite
• Reduced to tellurium
• Black (grey) colonies

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Nutrient Agar

• TSA
• Not selective
• Not differential
• Divide plate
• Swab environment
• Moisten swab
• Label source

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Sabourauds

• Selective
• Yeast
• Fungus
• /- Differential
• Acidic pH
• Divide plate
• Swab environment
• Moisten swab
• Label source

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Urine Culture

• TSA plate
• Calibrated loop (0.01ml)
• Clean catch sample
• Swab plate
• Confluent
• Isolation
• Calculate CFU/ml

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Normal Flora

• Results

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Snyder Deep Results

• CH2O fermentation
• Glucose
• Differential
• Rate of change
• Susceptibility for caries
• Selective for lactobacillus
• pH 4.8
• Indicator bromcresol green
• Green ? Yellow
• Dental caries
• Slight
• Mild
• Moderate
• Severe

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BAP results

• Not selective
• Differential
• Hemolysis
• Hemolysin exotoxins
• Types
• Gamma
• Alpha
• Beta

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Chocolate Agar Results

• Differential
• Growth
• Lysed RBCs
• CO2 capnophiles
• Oxidase Test
• Neisseria

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Oxidase Test

• Oxidase Reagent
• Cytochrome C in ETC
• Dark Purple positive
• Neisseria

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Oxidase Reaction
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MSA Results

• Selective
• Differential
• Results
• Fermentation
• acid end products
• yellow
• Components
• 7.5 NaCl
• Mannitol
• pH indicator phenol red

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MH-T Results

• Differential
• Diptheroids
• Mueller Hinton
• Nutrient agar
• Controlled proteins/ions
• Diffusion
• Tellurite
• Reduced to tellurium
• Black (grey) colonies

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Sabarouds Results

• Selective
• Yeast
• Fungus
• /- Differential
• Acidic pH
• 5.5
• acidophiles

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TSA Environmental Culture
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UTI

• CFU/ml
• colonies/0.01ml
• UTI
• gt 1x10(5)/ml
• Clinical signs
• Other urine tests