Cheek Cell DNA Extraction

1
Cheek Cell DNA Extraction
2
DNA Isolation

• Procedure
• Swish your mouth with Gatorade for one minute.
  Chew lightly on your cheeks. Be careful not to
  swallow the solution.
• Spit the solution back into the cup.

3
DNA Isolation

• Procedure
• 3. Carefully pour the spit solution into the
  15-mL plastic test-tube provided and screw on the
  cap
• 4. With a centrifuge, ask your teacher to spin
  the down your cheek cells for 30 seconds until it
  forms into a nice pellet at the bottom of the
  tube.

4
DNA Isolation

• Procedure
• 5. Use your pipette to carefully draw off most of
  the liquid on top, without disturbing the cell
  pellet in the bottom. Leave about 2 mL of
  liquid.

5
DNA Isolation

• Procedure
• 6. Using a different pipette, add 2mL of lysis
  buffer to your test-tube. The total amount of
  liquid in your tube should be 4mL.
• 7. Screw the cap back on and gently tilt the
  test-tube back and forth to mix the solution.

6
DNA Isolation

• Procedure
• 8. Using a different pipette, hold your 15mL test
  tube at a 45 angle, and drip the cold alcohol
  down the side of the test tube, so that it forms
  a layer on top of your solution without
  splashing. Do NOT mix.

7
DNA Isolation

• Procedure
• 9. Let the tube stand upright and undisturbed.
  You should begin to see clear strands of DNA
  clumping together in the alcohol layer.

8
DNA Isolation

• Procedure
• 10. With your pipette, carefully transfer the
  precipitated DNA along with about 1 ml of the
  alcohol solution into the small test tube that
  you will have for your necklace.