Kein Folientitel

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Summary of the current status of the work of
TUM-BO
Scientists Andreas Gattinger, Michael Schloter,
Alexandra Hagn (DNA), Ursula Bausenwein
(Socioeconomics) Technicians Franz Buegger
(EA-IRMS, GC-IRMS, GC/MS-c-IRMS, plant
labelling),Conny Galonska (DNA) Christine
Kollerbaur, Benjamin Mehnert (Lipids) Voluntary
worker (Environmental Protection) Matthias Weiss
(2003-2004), Jens Prescher (2004-2005) Technical
University of Munich(at the campus of
GSF-Research Center for Environment
Health)Chair of Soil Ecology, D- 85764
Neuherberg

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Summary of the current status of the work of
TUM-BO
1. Extraction and analysis of phospholipid
biomarker in peat (bog) samples (WP 04
D12-D14) 2. Extraction and analysis of DNA in
peat (bog) samples (WP 04 D12-D14) 3. Production
of 13C/15N labelled plant litter for field
experiment (WP 04 D13 WP 05 D19) 4.
Socioeconomical appraisal for German peatlands
(WP 01 D3)
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1. Extraction and analysis of phospholipid
biomarker in peat (bog) samples (W P04 D12-D14)
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Side chain analysis of phospholipids biomarker
to describe bacterial, eukaryotic and archaeal
diversity with particular emphasis on
methanogenic archaea and methanotrophic bacteria
the following fractions (biomarker) are analysed
Bacterial eukaryotic diversityAnalysis of
esterlinked fatty acids (PLFA)- saturated
(SATFA) Gram-positives, sulfate reducer-
monounsaturated (MUFA) Gram- negatives,
methanotrophs - polyunsaturated (PUFA) fungi,
protozoa
Archaeal diversity Analysis of etherlinked
isoprenoids (PLEL)- saturated short chain
(i200) all archaea - saturated long chain
(i400) all archaea - cyclic long chain
(i400-cy) Crenarchaeota- unsaturated short
chain (i201) methanogens
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Extraction and analysis of phospholipid
biomarker in peat (bog) samples (W P04 D12-D14)
From the peat samples investigated within work
programme 1, 208 samples were selected for PLFA
analysis from layer 6 and 8 only duplicate
samples were analysed to reduce sample amount for
PLFA and DNA analysis (59 from Finland (FI), 40
from France (FR), 46 from Switzerland (CH), 43
from Scotland (SCO), 20 from France (FB))
All PLFA samples are prepared 832 ( 360 from
labelling experiment) because of 4 different PLFA
fractions, in average 20-30 PLFA compounds per
run are to be identified and quantified
Problems with GC/MS-c-IRMS system since 4
months, company has not solved the problem yet
(GC columns of poor quality, splitting technique
unreliable, RF generator of the ion source is
unstable, compliance of the peak evaluation
software has not been sorted out)
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Simultaneous identification and quantification of
PLFA/PLEL from environmental samples and their
corresponding 12C/13C ratios by GC/MS-C-IRMS
20 of the analyte
MS(DSQ)
IRMS(DeltaPlusAdvantage)
80 of the analyte
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PLEL-derived isoprenoids (2-6 archaeal/methanogeni
c marker)
site comparison
sitesituation comparison
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PLEL-derived isoprenoids (archaeal/methanogenic
marker)
depthsituation comparison
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PLEL-derived isoprenoids (archaeal/methanogenic
marker)
depthsituation comparison
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2. Extraction and analysis of DNA in peat (bog)
samples (W P04 D12-D14)
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Reproducability
FI-A-3-3
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Heterogeneity
FI-A-3-2 FI-A-3-1
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Heterogeneity
FR-A-4-3 FR-A-4-1
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Depth profiling
FI-A-3-3 FI-A-2-1
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Depth profiling
CH-A-4-1 CH-A-3-4
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Extraction and analysis of DNA in peat (bog)
samples (WP 04 D12-D14)

• The same 208 peat samples were selected for DNA
  analysis as for PLFA
• From all 208 peat samples DNA was extracted (DNA
  extraction kit soilBio101 following test analysis
  with MLURI)
• MLURI (Rebekka) received all DNA extracts (apart
  from FB samples) for fungal community
  fingerprints
• EPFL/UfZ (Antonis) received DNA extracts (only
  CH samples) for protozoan diversity studies
• first DNA analysis by TUM-BO bacterial
  communities using 16S primer and subsequent
  t-RFLP analysis

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4. Socioeconomical appraisal for peatlands in
GermanyA. Gattinger, U. Bausenwein M. Drösler
(Uni Bayreuth)

• data on peatland distribution, economics on peat
  production, import/export has been collected
• in parallel a German group (among others M.
  Drösler, University of Bayreuth) is generating a
  new peatland inventory, as the current data is of
  poor quality (quite old, patchy, wrong, etc.)
• the major focus on the study will be on the
  political framework in Germany to support
  sustainable management of peatlands,
  reports/concepts from 3 of 6 selected German
  States (peatland area gt 2) on this issues have
  been received

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Hydrogenetic mire types in Germany
ombrogenous
geogenous
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31
3
Kesselmoore
17
5
14
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Distribution of mire types in Germany
Main mire type
1 coastal flood mires
3 water rise mires
2 coastal bogs
4 terrestrilisation mires
5 percolation mires
7 mountain bogs terrestrilisation mires
6 mountain bogs sloopy fens
8 water rise mires sloopy fens
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Occurence of mires gt 300 ha in Germany
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Distribution of European peatlands
total European peatland area 514 882 km2
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Usage of European peatlands
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Peatland use in Germany
18000
based on Lappalainen 1996 and Selin 1999
16000
mire
4
forest
13
12000
grass
52
km2
8000
4000
crop
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peat cut
2
0
bog
fen
totalpeatland
2002
total peatland area 13000 km2 ( 1.3 Mill.
ha) mire area 100 km2
peat is currently being formed
based on Freibauer et al. in prep
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Greenhouse gas budget
assuming a 100-year horizon
7000
12.0 of total Europe
Europe 51660 Gg
6000
60000
5000
50000
4000
40000
Gg CO2-equivalents
3000
30000
3.1
2000
20000
0.9
1000
10000
0.8
0.1
0
0
2nd largest emitter in Europe
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Greenhouse gas budget
assuming a 100-year horizon
7000
60000
6000
50000
5000
40000
4000
30000
3000
20000
Gg CO2-equivalents
2000
10000
1000
0
0
Europe 51660 Gg
-1000
-2000
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Socioeconomical appraisal for peatlands in
Germany

• for us the major task is to review the political
  framework regarding the sustainable management of
  peatlands (e.g. reduction of climate-relevant
  trace gases by converting arable field into
  extensive pastures/grasslands)