Single molecule probing of mechanical

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Single molecule probing of mechanical interactions
between exocytotic proteins Vladimir
Parpura Department of Cell Biology and
Neuroscience Center for Glial?Neuronal
Interactions University of California
Riverside Institute of Complex Adaptive Matter
Annual Conference Santa Fe, NM November 12, 2005
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Synapses are protoplasmatic kisses the final
ecstasy of an epic love story. Santiago Ramón y
Cajal
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Atomic Force Microscope (AFM)Basic Operation
MIRROR
LASER
PHOTODIODE
AFM CANTILEVER (DRIVEN BY
PIEZO)
AFM TIP
SAMPLE
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Force Spectroscopy
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B
40
30
Events ()
20
10
0
aa178-200 - - aa215-235 -
-
C
aa178-200
50
80
40
60
30
40
20
20
10
0
0
0
20
40
60
0
200
400
600
Number of events
Number of events
40
aa215-235
40
30
30
20
20
10
10
0
0
0
20
40
60
0
200
400
600
Extension (nm)
Force (pN)
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THANK YOU

• Umar Mohideen (UCR)
• Wei Liu
• Ed Chapman (UW)
• Jihong Bai
• Angel Garcia (RPI)

• Vedrana Montana
• Steve Rosenwald
• Yingchun Ni
• Erik B. Malarkey
• Xue Hua
• Vice unjara