References

1
Identification and molecular characterization of
actively expressed PERV LTR elements in Korean
pigs Jae-Won Huh1, Hong-Seok Ha1, Dae-Soo Kim2,
Byung-Wook Cho3, and Heui-Soo Kim1,2, 1Division
of Biological Sciences, College of Natural
Sciences, Pusan National University, Busan
609-735, Republic of Korea 2PBBRC,
Interdisciplinary Research Program of
Bioinformatics, College of Natural Sciences,
Pusan National University, Busan 609-735,
Republic of Korea 3Department of Animal Science,
College of Life Sciences, Pusan National
University, Miryang 627-706, Republic of
Korea http// www.primate.or.kr
Abstract
Results Discussion
Identification of actively expressed PERV LTR
elements
Porcine endogenous retroviruses (PERVs) in the
pig genome could be infectious risk factors in
xenotransplantation (pig-to-human). Long terminal
repeats (LTRs) of PERV element have promoter
activity which can affect the replication
properties of single PERV and adjacent genes.
Using the computational technique, we collected
all pig genome information including genome and
transcript sequences. Well conserved sequences in
LTR sequences are used for the identification of
actively expressed LTR elements Using the RT-PCR
approach, three different pigs (wild, domestic,
and hybrid with Yorkshire) are used for
amplification and cloning. About one hundred
individual clones were sequenced and analyzed.
Multiple alignment indicated that different
insertion and deletion (INDEL) and tandem
repeated sequences are located within the LTR
sequences. Transcriptional regulation of
different subtypes of LTR elements are
investigated by transient transfection assay,
indicating that five different types of LTR
elements are actively transcribed in pig genome.
Phylogenetic analysis of actively transcribed
PERV-LTR elements and clone information
Introduction
Transient transfection assay of PERV-LTR in
different cell lines
Large White
Korean domestic pig
Korean wild pig
RNA samples
RT-PCR
RT-PCR
Cloning
Sequencing
Analysis
References
Subtype identification
Cloning in Expression vector
Luciferase assay

1. Patience C, Takeuchi Y, Weiss RA (1997) Infection
   of human cells by an endogenous retrovirus of
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2. Scheef G, Fischer N, Krach U, Tonjes RR (2001)
   The number of a U3 repeat box acting as an
   enhancer in long terminal repeats of polytropic
   replication-competent porcine endogenous
   retroviruses dynamically fluctuates during serial
   virus passages in human cells. J Virol 75
   6933-6940

GENOME INFORMATION LABORATORY