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ACTIVE HETEROTROPHIC AND AUTOTROPHIC BIOMASS
DISTRIBUTION BETWEEN FIXED AND SUSPENDED SYSTEMS
IN A HYBRID BIOLOGICAL REACTOR   J. C. Ochoa1, J.
Colprim2,3, B. Palacios3, E. Paul1 and P.
Chatellier3
1 Laboratoire dingénierie des Procédés de
lEnvironnement (LIPE-EA833), Institut National
des Sciences Appliquées (INSA) Toulouse
(France). 2 Chemical and Environmental
Engineering Lab. (LEQUIA), EQATA department,
Universitat de Girona.(Catalonia-Spain) 3
Centre of International Research for Water and
Environment (C.I.R.S.E.E.), Lyonnaise des Eaux
(France)
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Introduction
Classical AS systems - Overloaded (flow or
charge) - Increasing environmental restriction
implementation/improving of N
removal Options - Adapt operational
conditions - Upgrade actual WWTP
Physically (adding a new treatment line)
Adding a support material (Hybrid
reactor) Hybrid reactor - How to measure active
fixed biomass (Biofilm) - Which is the biomass
distribution (fixed vs. suspended) interaction
fixed-suspended biomass - Which is the
composition (Autotrophic-Heterotrophic) growth
preferences of A-H biomass
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Objectives
Objectives Focused to an Hybrid Biological
Reactor Compared with a classical AS
system 1. Active Heterotrophic-Autotrophic
biomass determination 2. Evolution over time-SRT
reduction 3. Distribution between
fixed-suspended systems 4. Composition of
fixed-suspended systems
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Methodology

• Methodology
• Pilot plants

• Active biomass evolution with SRT
• 8 5 3.7 days SRT (CR)
• Effect of support filling tax
• HR from 20 to 10
• CR from 0 to 10

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Pilot plants I
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Pilots plants II
1. Control reactor (CR) Classical Activated
Sludge Process 2. Hybrid Reactor (HR) Plastic
support addition
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Operational conditions
Wastewater real, local sewer from Toulouse
(France), settled 30, Ref. 4ºC Mean values
Operational conditions - Continuous influent and
external recycle - Sequenced aeration (45 min.
45 min.) - Purge events. During anoxic periods
(first 15 min.) - External carbon source (at the
middle of anoxic period) - C/N 6-7 Applied
loading rate 0.9-1.2 Kg COD/m3d
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Active biomass I
Active biomass calculations (suspended and fixed
biomass) Close cell respirometer Samples
treatment Suspended biomass Centrifugation
refilling x3 Fixed biomass Support rinsed with
tap water (10 min) Oxygen saturation (up to 12
ppm)
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Active biomass II
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Results

• Biomass evolution (CR, HR)
• Autotrophic, XAR
• Heterotrophic, XHR
• Biomass distribution (Fixed vs. Reactor)
• Autotrophic, fAF/AR
• Heterotrophic, fHF/HR
• Total, fTF/TR
• Composition (Autotrophic fraction)
• Biofilm, fAF/TF
• Suspended, fAS/TS
• Total, fAR/TR

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Biomass evolution Performance

• Biomass evolution (CR, HR)
• Autotrophic, XAR
• Heterotrophic, XHR

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Biomass evolution Performance

• Biomass evolution (CR, HR)
• Autotrophic, XAR
• Heterotrophic, XHR

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Biomass distribution

• Biomass distribution (Fixed vs. Reactor)
• Autotrophic, fAF/AR
• Heterotrophic, fHF/HR
• Total, fTF/TR

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Composition

• Composition (Autotrophic fraction)
• Biofilm, fAF/TF
• Total, fAT/TR

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Composition

• Composition (Autotrophic fraction)
• Biofilm, fAF/TF
• Total, fAT/TR
• Suspended, fAS/TS

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Conclusions I
Conclusions

• Respirometric essays proved useful to calculate
  Active H-A biomass
• (Active) Biomass evolution
• XAR in HR is double than in CR
• XAR in CR reduced with SRT reduction
• when splitting colonised support, XAR similar
  in HR-CR
• XHR not function of SRT
• XHR in Hybrid R. more affected during Loading
  changes
• XHR in Control R. invariant with SRT- Loading
  changes
• Process performance
• COD removal Similar results, HR slightly
  better
• Nitrification
• CR depending of SRT
• HR High nitrifications levels at low SRT (3.03
  d)

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Conclusions II
Conclusions

• Biomass distribution (Hybrid Reactor)
• From 95 to 99 Autotrophic biomass is fixed
  (fAF/AR)
• About 60 Heterotrophic biomass fixed (fHF/AR)
• Composition
• Hybrid Reactor
• Biofilm 10-20 is Autotrophic
• Suspended less than 1 is Autotrophic
• Control Reactor
• SRT8 days. About 5 of Autotrophic biomass
• Clear reduction of Autotrophic biomass with SRT

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ACTIVE HETEROTROPHIC AND AUTOTROPHIC BIOMASS
DISTRIBUTION BETWEEN FIXED AND SUSPENDED SYSTEMS
IN A HYBRID BIOLOGICAL REACTOR   J. C. Ochoa1, J.
Colprim2,3, B. Palacios3, E. Paul1 and P.
Chatellier3
1 Laboratoire dingénierie des Procédés de
lEnvironnement (LIPE-EA833), Institut National
des Sciences Appliquées (INSA) Toulouse
(France). 2 Chemical and Environmental
Engineering Lab. (LEQUIA), EQATA department,
Universitat de Girona.(Catalonia-Spain) 3
Centre of International Research for Water and
Environment (C.I.R.S.E.E.), Lyonnaise des Eaux
(France)