Recombinase PowerPoint PPT Presentations

( Andrews et al., 1987; Hoess et al., 1984; Mack et al., 1992) ...

Cholangiocyte-specific Cre-recombinase transgenic mouse Arthur Kaser Dept of Medicine II Innsbruck Medical University Background Conventional knock-outs Can be ...

RAG=Recombinase activating gene defect (SCID) CD4. Btk. Ag. IL-2, 4, ... Recombinase activating gene (RAG-1 and 2) (T and B ... replication (109 viruses/day) ...

Directed gene expression in Drosophila (GAL4/UAS system) mcd. UAS. mcd. GAL4. Genomic Enhancer. Mosaic analysis using FLP recombinase ...

RAG-1/2 (Recombinase activating gene) deficiency: Required for TCR and Ig gene rearrangement ... virus replication (109 viruses/day) Live attenuated may result ...

Gene cassettes in integrons. Cre. Dimer reduction in phage P1 plasmids. XerC/D ... Can also catalyze inversion if recognition sites are oriented correctly, but not ...

What is the main dichotomy between innovation and caution (or risk, or the ... the outcross of transgenes, or minimize the production of transgene-encoded ...

and thus lead to a more Dyn-amic genome structure. Introduce ... its dual action as a stimulatory cofactor for excision and an inhibitor of ...

Cell Growth & Differentiation Vol. 10, 545-554, 1999 Inhibition of the Jun Kinase Pathway Blocks DNA Repair, Enhances p53-mediated Apoptosis and Promotes Gene ...

3.DNA cleavage via a transesterification reaction can also occur between two ... use this same site to catalyze both the DNA cleavage and the DNA strand transfer ...

Title: Author: zhanglixiu Last modified by: Default Created Date: 10/6/2002 1:08:11 AM Document presentation format

... the appearance in the fall of 1927 of George W. Beadle (a Nobel Laureate) at the ... Both Beadle and Rhoades recognized the need and the significance of exploring ...

The transposase first cleaves one DNA strand at each end of the transposon, ... inserts the transposon into target site using the DNA strand transfer reaction. ...

Title: PowerPoint Presentation Last modified by: Davidson College Document presentation format: On-screen Show Company: Davidson College Other titles

Weiss' Bacterial cell division and the septal ring' Review Article' Molecular Microbiology' 54 2004

Inactive allele. CRE. loxP site. Active allele. Cell-specific promoter. NEO ... Inactive allele. NEO. Targeting construct. mutation. CRE. loxP site. Active ...

Transgenic Mouse: Generic term for an engineered mouse that has a normal DNA sequence for a gene replaced by an engineered sequence or a sequence from another organism.

Chapter 15. Gene cloning and DNA analysis in agriculture Pyrethroids

Genome Engineering. Goal: Manipulate pieces of DNA to build recombinant ... Zebrafish -REMI -Transposase systems working and gaining popularity. Mouse ...

Deciphering HoxA9 function in multipotential progenitor biology and B cell development Kay L. Medina, PhD Associate Professor Dept. of Immunology

Title: Slide 1 Author: Michelle Last modified by: kumi2la Created Date: 4/16/2004 6:23:50 AM Document presentation format: On-screen Show Other titles

Bio 426. Knock-out, Knock-in, Knock-down. Dr. Thorsten Buch, Neuroimmunology ... tca tta ttc ctt agg tragttgtggttctrayytacagaaaagccagyyyyyncagg gta ttc agg ctg gat ...

Gain of function: transgenics, gene repair. Loss of function: (knockout, knockdown, ... (agouti) agouti black. Is this a useful chimera? YES!. Acknowledgements ...

Mouse DNA To Aid Biomedical Research. ... generation of mouse mutants ... 'North American COnditional Mouse Mutagenesis) Project ...

A knockout mouse refers to a laboratory mouse in which researchers have either inactivated or” knocked out” an already existing gene by replacing it or disrupting it by an artificial piece of DNA. The loss of gene activity often causes changes in a mouse phenotype that includes characteristics like appearance, behavior and other observable physical and biochemical characteristics.

Do flies have disease-gene homologs? ... Screens take more work in flies than in worms. Some things only possible in flies and not worms - physiology, some ...

... Departamento de Bot nica, Universidade de S o Paulo, S o Paulo/SP, Brasil ... elements associated with integrases genes act as key agents in the evolution ...

The amount of crossing over is minimal and only occurs ... Hybrid Dysgenesis. P-elements with hybrid dysgenesis serve as transformation vectors and as genetic ...

... genes from transgenic plants' P.D. Hare, NH Chua, Nature Biotechnology, 2002, p. 575-580 ... identification and cloning the gene of interest (unrelated ...

F factor and Conjugation. Conjugation is a process in which DNA is transferred from bacterial donor cell to a recipient cell by cell-to-cell contact

( A) Diagram of LM-PCR strategies for XmnI or EcoRV cleavage products. ... ( B and E) Levels of RE cleavage products within modified substrates. ...

Quality knockout is a sort of hereditary designing innovation for a certain however obscure capacity grouping, changing natural quality, as an aftereffect of particular loss of capacity to have further effect on the organism.With this sort of this method,we can construe the organic capacity of the quality.

Next lecture:techniques used to study the role of genes in develpoment Random genetics followed by screening Targeted mutagenesis (gene knockout)

Immunoglobulins Generation of Diversity

Title: Bacteriophages: basic characteristics, biology and diversity. Last modified by: M DS Document presentation format: On-screen Show Other titles

Cloning in S. cerevisiae (cloning in eukaryotes, part 1) Why clone in eukaryotes? Eukaryotic genes may not be expressed properly in bacterial host

http://www.classwire.com/Login (website for Text Book) ... http://www.whfreeman.com/kuby/ (has good self test) ... Note: Pepsin cleaves IgG into one (Fab')2 fragment. ...

E2A and acute lymphoblastic leukemias (ALL)

lysis: splitting open of the bacteria (ie. to release phage) ... 1 bacteria lyses, releasing 100's of phage, they infect nearby bacteria, etc ...

Addition and Deletion of Nucleotides. Diversity of the Hypervariable region 3 (CDR3) ... Deletions. Additions. Terminal deoxyribonuceotidyl transferase (TdT) ...

BBSRC MIBTP Site directed mutagenesis, reverse genetics and complementation Chris Thomas, Joanne Hothersall, Yusra Al-Sammarraie, Mukul Yadav Biosciences, University ...

Tricks to monitor Cre expression: ROSA26. ROSA = reverse orientation splice acceptor ... Tricks to monitor Cre expression: ROSA26R. Soriano, P. 1999. Nature ...

What is a Knockout Mouse? - a mouse in which a gene has been deleted/mutated ... inactivate gene = missing or non-functional protein - loss of function is assessed in ...

The expression of antigen receptor genes is the Key event in lymphocyte Development/Maturation ... Before Antigenic Exposure. How to code for different antibodies? ...

PCR primers. Avoid hairpins, homology at 3' ends, repetitive ... Arbitrary primer PCR. Gene fusion by PCR. Intermediate products. I II III. PCRs: Final product ...

The dopaminergic reward system The mesolimbic dopamine reward pathway is composed of dopamine cell bodies in the VTA that project to the NAc. The VTA also projects to ...

Promoters, Ribosome Binding Sites, Coding Sequences, terminators, Plasmids ... Taming Nature. Most parts are derived from natural systems. Building Devices ...

... et al inversion switch. Heritable inversion swith. State determined by sequence of inputs across ... Reporter records path traverse through states. Inversion ...

Wednesday, May 9

a) all will be variegated (both black and white patches of coat color) ... 6. You mate a variegated (chimeric) female mouse to a white male, and examine ...

Immunoglobulins Generation of Diversity * * 1. Rearrangements only occur between segments on the same chromosome. 2. A heptamer must pair with a complementary ...

What would be a likely phenotype of a mutation in the gene torso which ... If you treat an amphibian blastula with antibodies to block BMP-4 ...

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In case recombination does not happen at the desired locus, ES cells showing ... isolated from the P1 bacteriophage, catalyzes recombination between two of ...

Voltage-Gated Sodium Channels ... Kv4.2 Nav 1 increases Kv4.2-encoded current densities Coexpression with Nav 1 increases total and cell-surface Kv4.2 protein ...

... from 6-8 week old wild type Balb/c mice, from mice containing a floxed exon ... into the cleared fat pads of Balb/c mice 48h after transduction, and outgrowths ...