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RNA Lacks Functional Groups with a Neutral pKa ... Macon and Wolfenden, Biochemistry 7, 3453-58 (1968) Saito and Fujii, J. Chem. Soc. ...
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2 analogues (b and c) that should bind to the active site of PT on the ... Isoleucine tRNA synthetase (IleRS) discriminates 50,000-fold for Ile over valine ...
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50S Ribosome Subunit. X-ray Structure. 50S Ribosome Subunit. Peptidyl Transferase ...
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(Lost in) Translation Briefly review prokaryotic machinery Initiation in Eukaryotes Where in the world is Peptidyl Transferase? tRNA charging: The second code
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ROLES OF IF-1 AND IF-3 IN RIBOSOME DISSOCIATION ... CHLORAMPHENICOL: Inhibits peptidyl trans- ferase on prokaryotic 50S subunit ...
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Chloramphenicol. Binds 23S rRNA on 50S subunit. Inhibits peptidyl transferase activity ... blood dyscrasia (chloramphenicol) hemolytic anemia (sulfonamides) ...
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... is not quite unambiguous: in E. coli, selenocysteine is introduced into formate ... Peptide bond formation is catalyzed by peptidyl transferase (historical name) ...
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the initiation codon is recognized. eIF2 dissociates from the complex ... the ribosome shifts one codon along. the mRNA, moving peptidyl tRNA ...
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How does the mRNA know where to bind. and where will ... UAA, UAG and UGA are stop signals. Chain termination. Release factors GTP. Peptide is released. ...
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Title: PowerPoint Author: lhs Last modified by: chen Created Date: 8/18/2000 3:09:50 AM Document presentation format:
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Ribosomal RNA Phillips Huang BMS 265 rRNA Nomenclature Named based on sedimentation rates, measured in Svedberg units (S) Larger sedimentation coefficients indicate ...
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Protein Biosynthesis II mRNA Binding Shine-Dalgarno Sequence Initiation Complex f-Met-tRNAfMet Initiation factors Elongation Elongation factors
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If We want to learn about the nature of elongation,We would ask: ... 50S subunits are inhibited by carbomycin(???), chloramphenical(???), and RNase ...
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Formation of the Ternary Complex of GTP, Aminoacyl-tRNA, and EF-Tu ... EF-Ts Is Only Necessary For Ternary Complex Formation When Starting From EF-Tu-GDP ...
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Title: PowerPoint bemutat Author: Genetikai Int zet Last modified by: K hidai L szl Created Date: 9/20/2001 8:41:55 AM Document presentation format
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Title: Introduction to the study of cell biology Author: Zhouyong Last modified by: Created Date: 9/7/2002 1:34:05 PM Document presentation format
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Quality control in ER N-X-S/T N N- folding, targeting
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The translation complex is assembled at the beginning of the mRNA coding sequence ... A preinitiation complex forms (40S ribosome, aminoacylated initiator tRNA, other ...
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Chapter 12 Translation
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Electron Micrograph of a DNA-RNA Hybrid. tRNA Transcription & Post Modification ... requires mRNA, ribosomes, tRNA, aminoacyl tRNA synthetases, and initiation, ...
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Translation proteins (polypeptides ...
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Protein Synthesis One Gene One Enzyme Protein Synthesis Protein Synthesis Protein Synthesis Protein Synthesis Protein Synthesis Protein Synthesis Transcription ...
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Rate-limiting (most regulated) step is usually initiation. ... Central protuberance. stalk. ridge. head. stalk. platform. platform ...
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Title: Inquiry into Life Twelfth Edition Author: Administrator Last modified by: user Created Date: 8/8/2006 9:50:44 PM Document presentation format
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Structure and Pharmacophore Elucidation of the HRV14-3C Protease. Trent Bjorndahl ... 1Rates of peptide hydrolysis are expressed relative to 2C-3A and are the mean of ...
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Biochemistry Chen Yonggang Zhejiang University Schools of Medicine Bodega Bay, Sonoma County Breakfast at The Tides, Bodega Bay The process of using base pairing ...
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Frameshift mutation: add or delete base (X) Without code gaps: AUX GCA GCC AAC ... not tightly bound to ribosome, so released soon translation aborted ...
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Components needed for Translation tRNAs Aminoacyl-tRNA synthetases Ribosomes
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Puromycin resembles tyrosyl-tRNA, binds to the A site, accepts peptide from ... Central protuberance. stalk. ridge. head. stalk. platform. platform ...
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Crick's Wobble Hypothesis argues for the second possibility - the first base of ... Advantage of wobble: dissociation of tRNA from mRNA is faster and protein ...
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Some other sRNAs are known to repress translation intead of activating ... Binding of TPP represses translation of mRNAs that encode enzymes involved in TPP synthesis ...
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Title: Protein Synthesis Author: McIntyre Last modified by: Tara and Eric McIntyre Created Date: 10/7/2003 9:46:27 PM Document presentation format
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Most modified chemically from original compounds found in nature, some isolated ... catalog/ProductDetail.do?N4=A1593|SIAL&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPE C ...
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... {EC 1.11.1.9}, tetraiodothyronine 5' deiodinases,thioredoxin reductases, formate dehydrogenases, glycine reductases a n kter ch hydrogen z ch).
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Basic protein structure and stability II: Topics in side chain and backbone chemistry ... Some other important of the chemistry of side chains will also be ...
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DNA later took over the genetic role and proteins took over the ... The RNA world arose de novo in the form of self replicating ribozymes. Almost certainly true ...
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Title: PowerPoint Author: VAIO Last modified by: pharm Created Date: 10/3/2003 1:43:49 AM Document presentation format:
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intracellular catabolism of peptides and proteins. processing of prohormones and proenzymes. penetration of normal human tissue by malignant cells and possibly ...
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2. Beadle and Tatum (Neurospora mutants) C. rRNA vs mRNA as the molecule for protein information storage ... Beadle and Tatum. George W. Beadle. Edward L.Tatum ...
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Chapter 14 Protein Synthesis. Some key words in this chapter: Ribosome (???) ... binds ATP and the correct amino acid (based on size, charge, hydrophobicity) ...
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... are mainly made from RNA, and adapter molecules (tRNA) are exclusively made RNA. ... In fact, tRNA looks like Nature's attempt to make RNA do the job of a ...
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IX: DNA Function: Protein Synthesis A. Overview: B. Transcription: C. RNA Processing: D. Deciphering the Genetic Code E. Translation!!! 1. Players: 2.
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... Protein Synthesis * * Figure 15.3 Method used to determine the ... biochemical pathway leads to ... PowerPoint Presentation Author: myself i Last ...
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HINTS on using. literature databases. Louis Volkers & Gerdien de Jonge ... automatic check for new citations by e-mail. special filter selections. Actions: ...
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20 enzymes to activate amino acid precursors 12 proteins/enzymes for the initiation, ... Ribosomal subunits are identified by their S (Svedberg unit) ...
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cis-acting hydrolase element (
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IX: DNA Function: Protein Synthesis A. Overview: B. Transcription: C. RNA Processing: D. Deciphering the Genetic Code E. Translation!!! 1. Players: 2.
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LECT 20: PROTEIN SYNTHESIS AND TRANSLATIONAL CONTROL High fidelity of protein synthesis from mRNA is essential. Mechanisms controling translation accuracy include ...
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Deletion of the three underlined nucleotides (UAU) in the ... Which of the following enzymes is a target of the antibiotic erythromycin? A. beta-lactamase ...
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Colinearity of Gene and Protein DNA genotype DNA sequence transcription RNA translation amino acid sequence protein function organism phenotype Molecular Basis for ...
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Title: Chapter 12. Protein biosynthesis (P215, sP875) Last modified by: Created Date: 5/27/2004 12:36:25 PM Document presentation format
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... the aminoacyl adenylate to the cognate tRNA, but hydrolyze the aminoacyl ... 3-4 tRNA synthetases recognize unique structure features of cognate tRNAs ...
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RNA strand scission. RNA splicing. Peptide bond ... RNA strand scission ... and ribozymes catalyze phosphodiester bond scission by a variety of mechanisms ...
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Humans share most of the same protein families with worms, flies, and plants Hair grows by forming new cells at the base of the root. As they move upward through the ...
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