Microarrays PowerPoint PPT Presentations

Semester project: Microarrays and Statistics Part 2 of 2: Introduction to Microarrays

Microarrays

DNA in somatic cells, all cells except the gametes, is the same ... disease-free based on both clinical and radiological testing. ... (digital camera) ...

Removal of outliers. Absolute measurements. cDNA microarray ... Can we make better treatment decisions for a cancer patient based on gene expression profile? ...

The conentration of labels in the cDNA is measured! ... gar. Gene 6. 0.86. 0.84. 0.42. 0.84. 0.44. groo. Gene 5. 0.41. 0.21. 0.11. 0.26. 0.60. bas. Gene 4 ...

Protein microarrays Martyna Franczuk Functional protein microarrays differ from analytical arrays in that functional protein arrays are made of full-length functional ...

These antibodies can be made in large quantities and have a specific affinity ... the Methodist Hospital Houston, TX, USA, prior to commencement of radiotherapy. ...

'Spot' them on a medium, e.g. an ordinary glass microscope ... Spot localization and detection, including the extraction of the background ...

Experimental Gerontology 40: 737-744 (2005) ...

'A microarray is a tool for analyzing gene expression that consists of a small ... Pyxis CattleArray 7600. Bovine total leukocyte cDNA library & immune gene amplicons ...

DNA Microarrays O que DNA Microarray? Tamb m conhecido como chip de DNA Permite a medida do n vel de transcri o de cada gene no genoma (express o dos genes ...

13 week-old C57BL/6 mouse (Ovariectomy 11week-old) Soy-free diet for 1 week. 100 mg /kg x day ... Harvest. organ. Total RNA. Isolation. Affymetrix GeneChip ...

Protein Lysate Microarrays Clay Scott Ryan McConnell Shannon Neeley

V7 Genexpression - Microarrays Idee: analysiere Ko-Expression von mehreren Genen um auf funktionelle hnlichkeiten zu schlie en wichtige Fragen:

Databases for Microarrays Vidhya Jagannathan SIB, Lausanne Overview Microarray data in a nutshell Why databases? What data to represent? What is a database?

Epistasis Analysis Using Microarrays Chris Workman

Printing High Quality Microarrays

Lysis: The dissolution or destruction of cells by the action of a specific lysin ... cellular debris and fluid produced by lysis. Protein Lysate Array Design ...

Probe selection for Microarrays Considerations and pitfalls

Title: Analysis of Affymetrix Microarrays Author: Gareth Palidwor Last modified by: gpalidwor Created Date: 1/18/2005 5:20:25 PM Document presentation format

Basics on microarrays. Concepts of cDNA, mRNA, dyes, probes, hybridization, ... How to resolve the errors and bias introduced by the microarray experiment ...

Introduction to Affymetrix Microarrays Why use microarrays? Permits expression profiling of thousands of genes in parallel Why use Affymetrix microarrays?

... (hybridize), and labeled DNA will be detected on photographic film ... Correct bias in MA plot for each print-tip. Correct bias in MA plot for each sector ...

DNA chip drawbacks: Possibly provide too much data ... DNA microarray drawbacks: Do not measure as great a variety of hybridization ...

Title: The Impact of the Human Genome Project on Clinical Diagnostics Author: Mount Sinai Hospital Last modified by: SaRa OrTeGa Created Date: 11/27/2000 1:47:21 PM

Uses of Microarrays in Research Anne Rosenwald Georgetown University Microarrays in Research: A Survey of PubMed Recent Microarray Papers: I. New Techniques ...

Other uses of DNA microarrays

Human Genome Project. By Dept of Energy and NIH and. Celera Genomics ... The Stanford microarray resources: http://genome-www5.stanford.edu/resources/ A flash ...

Gene is expressed by transcribing DNA into single-stranded mRNA mRNA is later ... expression can be measured with latest technology mRNA is isolated and ...

One base at a time. Uses process of photolithography. Developed for printing ... Composition based on design rules. Empirically derived. Photolithography ...

Introduction to DNA Microarrays Todd Lowe BME 88a March 11, 2003 ... Genomic DNA -- similarity of genomes Genetic Footprinting Species cross hybridization ...

Transcription is a complex process involving several steps and many ... element and TATA box, usually lying just upstream (at the 5' end) of the coding region ...

MOLECULAR NEUROENDOCRINOLOGY RESEARCH GROUP. BIOMEDICAL DISCOVERY USING MICROARRAYS ... MOLECULAR NEUROENDOCRINOLOGY RESEARCH GROUP. FUNCTIONAL GENOMICS. 30, ...

Exploring the Limitations of DNA Microarray Technology: A Case Study in Quality Control Emily Oldham1, 2, A. Malcolm Campbell1, Laurie J. Heyer 2

Gene Regulation and Microarrays ...after which we come back to multiple ... Measuring gene transcription in a high-throughput fashion. What is a microarray ...

1. Ch 8. Chapter 8 (Addedum) - Microarrays. Microarrays. 2. Ch 8. What are ... Spot containing short segment of a gene. 3. Ch 8. How to set up a microarray: ...

... microarrays for allergen standardization. 2 /37. How do we measure potency? Total protein (hymenoptera) Overall allergen (grasses, mites) Pooled human antibody ...

Microarray gene expression profiling. o design and analysis techniques. o example: ... in situ through an ink-jet process (Rosetta Inpharmatics, Kirkland WA) ...

DNA Sequencing and Microarrays Janelle Dy, Cameron Kneib, Bailey Robinson History of Sequencing 1973- Walter Gilbert and Allan Maxam sequence 24 bp 1975- Frederic ...

Gene expression and DNA microarrays

Significance analysis of microarrays ... multi-class censored survival one ... Rank the permuted d-values of the genes in ascending order v) Repeat steps iii) ...

Microarrays in Undergraduate Research. Group ... white bar indicates gene selected by hand (ORC2) -redder color indicates higher expression in dna2-1 dna2-1 WT ...

Uses of Microarrays in Research Anne Rosenwald Biology Department Georgetown University Microarrays in Research: A Survey of PubMed Recent Microarray Papers: I.

Introduction to Bioinformatics. Bio-nanotechnology: miniaturization and automation ... Bioinformatics in MD Anderson Cancer Center. An award winning team: ...

CS 6463: (J) Introduction to Microarray. 1. K. Further Applications of Microarrays ... Investigators at Rosetta Inpharmatics look at yeast microarray notice that 4 ...

Download normalised M values (txt file) Microarray Data Flow at TIGR. Image Analysis ... Requires Java Runtime environment and Windows XP. MIDAS data analysis methods ...

Department of Statistics, University of California, ... Transfer to Oak Ridge phenol resistant centrifuge tubes. 3. Add 800 ul 25% SDS, 12 ml acid phenol. ...

... greater than 91% homology between chick brain, mouse brain, and human ... A single polyadenylation site in the chick clone was also present and duplicated ...

Title: Assessment of the reproducibility of gene expression profile in a breast cancer cell line Author: Lara Lusa Last modified by: lara Created Date

Microarrays were developed and commercialized in the eighties. Since then, market has offered tremendous growth and hence has seen an influx of players vying fiercely for a share or this market. The Global DNA & Gene Chip (microarray) market was valued at $760 million in 2010 and is expected to reach $1,425.2 million by 2015 growing at a CAGR of 13.4%. Major players in this market include Affymetrix, Inc. (U.S.), Illumnia, Inc. (U.S.), Agilent Technologies, Inc. (U.S.), Roche NimbleGen (U.S.), Sequenom Inc, (U.S.), and others.

Introduction to Bioinformatics Microarrays1: Microarray Technology Course 341 Department of Computing Imperial College, London Moustafa Ghanem Aims for the 2nd part ...

Thousands of genes are each ... the decision about where to create branches and in what order to present them ... J. of Cell Science 113, 2000, 4151-4156. ...

MAGE. Computer standard. A standard 'object model' for Microarrays ... MAGE-ML. A collection of these objects in an XML format ...

Missing value estimation methods for DNA microarrays Statistics and Genomics Seminar and Reading Group 12-8-03 Ra l Aguilar Schall Introduction Missing value ...

ORFs are defined by an ATG start codon followed by enough. bases before a stop codon to indicate that there is a potential. gene (called 'putative gene' ...

Class prediction for experiments with microarrays Lara Lusa In titut za biomedicinsko informatiko Medicinska fakulteta Lara.Lusa at mf.uni-lj.si