Methods%20in%20Enzymology%20????(??) - PowerPoint PPT Presentation

Title: Methods%20in%20Enzymology%20????(??)


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Methods in Enzymology????(??)
Aily Qin
2
??????????
3

1. ????,????

• ???1955?,?????60?????
• ?1 - Preparation and Assay of
  Enzymes(???????),?????????????????
• ?????,????????????????,????????????,??????????????
  ??????
• ?????????????????,????????????????????????
• ??50,000????????,?????????????
• ?ScienceDirect?????????????

4
2. ?????

• ?????????????,?????????????????????

• ??????????????????????????

5
3. ?????

• ???????????????,??????????????

• DNA,RNA,???,???
• ???(????????????)
• ?????,????,??,???
• ?????,???,????
• ???????????,??,?????,???
• G??????,????????????
• ???????,????,?????
• ??????,??
• ?????,?????????,????
• ????
• ????,???
• ???,????,???,???
• ????,?????
• ..

6
4. ????????????

• ?????????????????

• ??????,????,??????
• ???,????????????,RNA??,????,????
• ???????,??????????,
• ????
• ????
• ??????????
• ????????????,????,??????,???????
• ????,????,????,????,?????
• ??????,??
• ?????,????,????????,???-???????
• ???????????
• ?????????
• ??????????
• ??????????,???
• .

7
5. ????????????

• ???????????,?????????????????????????
• ?2015?1?1??????,??????7??
• ?????????????????,???????????????????
• ???????????????????????????

8
??????????
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???Volume 58 - Cell Culture ????
Culture cells in vitro simulating in vivo
environment
cell culture medium
cell culture incubator
Cell culture dish
Sterile work table
Pipette
Microscope
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???Volume 58 - Cell Culture ????
applications
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???Volume 58 - Cell Culture ????

• ?????????,?????????????????????????

Part 1
Part 2
Part 3
????????????????
??????????????
??????????????????

• ????????
• ??????
• ???????
• ????????
• ???????
• ???????????????
• ..

• ?????????
• ?????????
• ????
• ????
• ?????????
• ????????????
• ?????
• ???????????????
• ..

• ?????
• ???
• ?????
• ?????
• ???????
• ???????
• ?????????
• ????
• ?????????????

12
???Volume 101 - Recombinant DNA ??DNA
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???Volume 101 - Recombinant DNA ??DNA
GMO

• genetically-modified organism

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???Volume 101 - Recombinant DNA ??DNA

• ??DNA???????????????????????????????,???????????,?
  ?,?????????,??????
• ????????????????(GMO)??????????DNA??
• ???????DNA????????DNA??,??,??,??,???????????????
  ???????,??????????????????
• Volume100,101,153,154,155-Recombinant DNA
  ??????DNA????
• ??Volume 101????????????Sidney
  Brenner(2002?????
• ??/???)? Andrew Fire(2006???????/???)?
• ????????20????DNA???,???????????????????????

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???Volume 152 - Guide to Molecular Cloning
Techniques ????
16
???Volume 152 - Guide to Molecular Cloning
Techniques ????

• ????DNA?????,????????????????????????,????????????
  ???,?????????????,????????????????????????
• ??????????????,????????????
• ?????DNA?????????,???????????,?????????
• ??????????????
• ????????????????????,?????????????????????????????
  ?????

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???Volume 182 - Guide to Protein Purification
???????
Purified protein
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???Volume 182 - Guide to Protein Purification
???????

• DNA??????????????,???????????????????????????????,
  ???????????????????????,??????????????????,???????
  ????????????????
• 2010?,?Richard Burgess????Murray
  Deutcher????,?????????????????????????

• ????????,?????
• ??????,???????????
• ?????????
• ?E.coli?????????????????
• ??????
• ???????,??????,??????,????????
• ??????,????,????,??????
• ???????????,??????
• ???????????
• ????????
• ?????
• ?/????????,????????,?????
• ?????????
• ?????????????????

????????????
Volume 182
???????????????
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???Volume 207 - Ion Channels ????

• ?????????????????,??????????????,????????????,????
  ?????????????????????????????????????

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???Volume 207 - Ion Channels ????

• ??????Erwin Neher(1991???????/???),?????????????
  ??
• ????????????

• ???????????,??
• ?????????
• ???????????

• ?????????????????,????????,????,?????,????????????
  ?????????????????????

21
???Stem Cells ???

• ????????????????

• ??????????????????,???????????????????????????
• ?????????????????

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???Stem Cells ???

• ??2003??????Volume 365-Differentiation of
  Embryonic Stem Cells ????????????????????????
• 2006?-2007????????????
• Volume 418-Embryonic Stem Cells ?????
• Volume 419-Adult Stem Cells ?????
• Volume 420-Stem Cell Tools and Other
  Experimental Protocols
• 
  ?????????????
• ???????????review???????????,????????????????????
• ???????????????????,?????????????,????????????????
  ?????????????

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????????????????

• ???????????????????????????
• Volume 529-Laboratory Methods in
  Enzymology DNA
• Volume 530- Laboratory Methods in
  Enzymology RNA
• Volume 533- Laboratory Methods in
  Enzymology Cell, Lipid and Carbohydrate
• Volume 536539541-Laboratory Methods in
  Enzymology Protein
• Volume 537538 - Methods of Adipose Tissue
  Biology
• .

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????ScienceDirect ??www.sciencedirect.com

• ???????????????????
• ???????????????20-25???

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?2000?????????
?2000????,?????????6693??? ????3819???????2270??
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?2000?????????

• ?????????????????Cell,Science,Nature???????83,271?
  ???
• ????????????????????????????

???????????? ?????????83,000??
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?2000?????????
????????136,821? ????
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