Title: Free Radical Scavenging Activity of Some Malaysian Medicinal Plants
1Free Radical Scavenging Activity of Some
Malaysian Medicinal Plants
Faculty of Pharmacy, International Islamic
University Malaysia
19th CPA-MPS 2007, KUALA LUMPUR
2Presentation layout
- Introduction
- Objective
- Methodology
- Results and discussion
- Conclusion
3INTRODUCTION
4Antioxidant
- Any substance, when present at low
concentrations compared with those of an
oxidizable substrate, significantly delays or
prevents oxidation of that substrate
Free Radicals in Biological Medicine, 3rd ed. 1999
5Antioxidant action
- The antioxidant can act by the following
mechanisms reducing activity, free
radical-scavenging, potential complexing of
pro-oxidant metals and quenching of singlet
oxygen.
6Effect of free radical
- Tissue damage resulting from free radical may
cause a variety of disorders, including
degenerative disorders of the CNS, such as
Alzheimers disease, cancer, atherosclerosis,
diabetes mellitus, hypertension, AIDS and aging
- (Halliwell and Gutteridge, 1998 and Mantle et
al., 2000). -
7Plant materials
- Andrographis paniculata (leaves),
- Averrhoa belimbi (fruits),
- Eurycoma longifolia (roots),
- Dipteris lobbiana (leaves),
- Ficus deltoidea (leaves),
- Fagrea obovata (roots),
- Momordica subanguenta (fruits),
- Morinda citrifolia (fruits),
- Orthosiphon stamineus (leaves),
- Smilax myosotiflora (rhizome)
8Traditional Uses
- Aphrodisiac and energy enhancer
- Eurycoma longifolia, Smilax myosotiflora,
Dipteris lobbiana, Ficus deltoidea and Fagrea
obovata - Antidiabetes mellitus
- Momordica subanguenta, Orthosiphon stamineus,
Andrographis paniculata, Averrhoa belimbi and
Morinda citrifolia
9Eurycoma longifoliaTongkat Ali/Malaysian ginseng
- Traditional uses tonic, poultice for headaches,
wounds ulcers, febrifuge, hepatitis, aphrodisiac
10Andrographis paniculataHempedu bumi (bile of
the earth), akarcerita
- Traditional uses Roots and leaves used against
snake bites, decoction of leaves against
stomach-ache, dysentery, typhus and bronchitis,
as a poultice on swollen legs, vitiligo, piles
and antidiabetes
11Orthosiphon aristatusMisai kucing, kumis kucing,
ruku hutan
- Traditional uses diabetes and kidney related
diseases, diuretic, regulate blood pressure
12Morinda citrifolianoni, mengkudu
- Traditional Uses to treat diabetes, haemorrhages
and coughs.
13Objective
- The research is aimed to study the relationship
of traditional usage of medicinal plants and
their free radical scavenging activity
14METHODOLOGY
15- 1. Extraction
- 50 g of powdered plants were extracted with
EtOHWater (5050) (500 mL) and freeze dryed - 2. Phenolic contents was determined by
Folin-Ciocalteu reagent (McDonald et al., 2001) - 3. Flavonoid contents was determined by aluminum
chloride colorimetric method (Siddhuraju and
Becker, 2003)
164. Free radical scavenging activity was
determined by 2,2-diphenyl-1-picrylhydrazyl
radical (DPPH ) scavenging
a. Spectrophotometric assay
- X Absorbance of sample at 517 nm
- Y Absorbance of control at 517 nm
b. Thin layer chromatography test (yellow
spots against purple background)
17RESULTS AND DISCUSSION
18Table 1. Percentage of soluble materials
extracted from the plants
Sample (Abbreviation) Local name Yield ( w/w)
Averrhoa belimbi (AB) Belimbing buloh 39.8
Fagrea obovata (FO) Tengkuk biawak 24.3
Dipteris lobbiana(DL) Janggut ali 17.9
Momordica subanguenta (MS) Peria katak 16.6
Andrographis paniculata (AP) Hempedu bumi 14.4
Orthosiphon stamineus (OS) Misai kucing 11.4
Ficus deltoidea(FD) Mas cotek 8.9
Smilax myosotiflora (SM) Ubi jaga 7.9
Morinda citrifolia (MC) Mengkudu 7.7
Eurycoma longifolia (EL) Tongkat ali 2.8
19Table 2. Total phenolic and flavonoids contents
of the samples
Sample Total phenolic (mg /g) Total flavonoid (mg/g) DPPH
Andrographis paniculata 43.58 2.47 19.0
Averrhoa belimbi 40.68 14.88 14.8
Eurycoma longifolia 53.75 3.37 24.0
Dipteris lobbiana 54.92 81.75 92.0
Ficus deltoidea 53.93 42.45 61.1
Fagrea obovata 54.58 74.13 91.6
Momordica subanguenta 48.30 0.15 22.9
Morinda citrifolia 53.75 11.80 46.1
Orthosiphon stamineus 54.54 56.75 87.5
Smilax myosotiflora 54.63 16.19 32.6
20Scavenging activity
ApAndrographis paniculata, AbAverrhoa belimbi,
ElEurycoma longifolia, DlDipteris lobbiana,
FdFicus deltoidea, FoFagrea obovata,
MoMomordica subanguenta, McMorinda citrifolia,
OsOrthosiphon stamineus and SmSmilax
myosotiflora
21Flavonoid contents and scavenging activity
ApAndrographis paniculata, AbAverrhoa belimbi,
ElEurycoma longifolia, DlDipteris lobbiana,
FdFicus deltoidea, FoFagrea obovata,
MoMomordica subanguenta, McMorinda citrifolia,
OsOrthosiphon stamineus and SmSmilax
myosotiflora, Que Quercetin
22TLC test
23Conclusion
- There is correlation between flavonoid content
and radical scavenging activity. - F.obovata, D.lobbiana and O.aristatus exhibited
highest free radical scavenging activity.
24THANK YOU