Introduction%20to%20the%20Microscope

1
Introduction to the Microscope

• Care
• Parts
• Focusing

2
Microscope Care

• Always carry with 2 hands
• Only use lens paper for cleaning
• Do not force knobs
• Always store covered
• Keep objects clear of desk and cords

3
Parts of a Compound Microscope
4
Microscope Parts
Eyepiece
Body Tube
Revolving Nosepiece
Arm
Objective Lens
Stage
Stage Clips
Coarse Focus
Diaphragm
Fine Focus
Light
Base
5
Compound Microscope

• A microscope is a very powerful magnifying glass
• A microscope helps you see things like cells up
  close

6
Eyepiece

• View the specimen through the eyepiece

7
Stage Clips Objectives

• Stage clips hold the slide in place
• Low power objective is used to focus the
  microscope (short fat)
• High power objective is used to view details of a
  specimen

8
Coarse Adjustment, Fine Adjustment, Base

• Coarse adjustment focuses adjustment
• Fine adjustment fine tunes gives detailed
  focus(usually smaller than coarse adjustment
  knob)
• Base is where the microscope rests

9
Stage

• Stage is part where the slide rests
• Mirror (or light source) directs light upwards
  onto the slide.

10
Diaphragm

• Diaphragm allows light in

11
Nosepiece

• Nosepiece is the rotating device that holds the
  objectives (lenses)

12
Arm

• Arm is the part where you carry the microscope

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Can you name the parts of a compound microscope?
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Answers

• base 
• mirror (light source)  
• diaphragm  
• stage  
• stage clips
• low power objective lens  
• high power objective lens  
• nosepiece  
• arm 
• fine focus knob  
• body tube  
• coarse focus knob  
• eyepiece

15
Types of Microscopes
16
Light microscope

• (for small objects - similar to the way
  binoculars magnify objects far away.)

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The Compound Light Microscope
http//www.cas.muohio.edu/mbi-ws/microscopes/comp
oundscope.html
18

• May have many lenses that magnify the object
• Eyepiece 10x magnification
• Objective 10x (low) or 40X (high)

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• Total magnification
• eyepiece X objective
• ex 10x X 40x 400x total magnification

20
Compound Light Microscope

• Powered by Light Lenses
• Powers of Magnification
• Ocular Lens _________
• Low Power Objective _______
• Medium Power Objective _______
• High Power Objective _______
• Calculating Total Magnification ____________
• Resolution
• Parts

21
Stereomicroscope

• To look at large things that light cannot pass
  through (one eyepiece for each eye)

22
Stereomicroscopes
http//scientificsonline.com/product.asp_Q_pn_E_31
19600
http//www.martinmicroscope.com/MicroscopePages/St
ereomicroscopes.htm
23
Stereomicroscopes

• 3 - D image
• can only magnify 10x to 600x

24
Electron Microscope

• can magnify more than 500,000x

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House Fly

• http//www.pbrc.hawaii.edu/bemf/microangela/hfly.h
  tm

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Using the Microscope

• Place the Slide on the Microscope
• Use Stage Clips
• Click Nosepiece to the lowest (shortest) setting
• Look into the Eyepiece
• Use the Coarse Focus

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Using High Power

• Follow steps to focus using low power
• Click the nosepiece to the longest objective
• Do NOT use the Coarse Focusing Knob
• Use the Fine Focus Knob to bring the slide into
  focus

What can you find on your slide?