Title: a??????:
1??? ?????????
??
1????????????,????????????????????????????????
??????????????????????????????
2????????????????????????????????????????????????
???
2????
??????? ???? ??? ???????????????,???????
??????? ??????,????????????,???????????????
?,???????????
3???????????????????????????????
????
?????????????,???????????????,????????,?????,?????
?????,??????????????
4????
?????,???????????????????????,???????????,????1-2?
??????????,??????????????????
5????
?????,??????,????????????,????????????????????????
??????????,????????????,????????
6????
?????????????????????????????????????????????????
??,??????????????????????
7???
??????????,???????????????????,???????????????????
,????????????,????????
8?????? 1?????????????????,????????,?????????
???? 2????????????,?????????????,?????????
3??????,???????????????????
9?????? 1?????,????????
2?????????,???????????? 3???????,??????,?????
0-4 ???,???? 4????????,??????
10????????
1?????? 2??? a?????? b??????(?????)
c?????? d??????
113??? a????? b???????????????(???????,??
????????????)
124????? ?????????????,?????????????,??????????
?,???????????? a????? b????? c?????
d?????? e??????
135???????????????????????????? a?????
b????? c????? d????? e?????
f????? ?????
14????(???) ????????????????????
???? 1?????????,???????????????
2??????????,???????????????,????????,???????
15(No Transcript)
16?????????(Agrobacterium tumefaciens)
??,1-4?????? ??????? ????22?
Ti??(Tumor inducing plasmid)??????
T-DNA??????????????????????
17?????? 1????????????????
2????????????????????,????????????????
3????????,??????????? 4?????????????????????
???? 5?????????,??????????????
6??????????????,????????????
18?????? 1???????? 2?????????????????
3????,???????,?????????75??????
4????????,????? 5????????????
6?????????????K84?
19????? ??????? ???? ?????????????????,????
????????,???????
????????????????????
20???? Meloidonyge spp.???,??????????
?????????? ??????
?????? ?????? ?????????????????
21?????? 1????????????????????????????.
2??????????????????????
? ???? ???? ????
???? ??
223???????? 1)???????? 2)??????????
3)?????????,?????2???????????????
23?????? 1???????,?????? 2????????
3????? ??????????????D.D???????
??????????45?????30-60min?
24 ????
- ???????
- ????
- ????????????????,???????????
- ???????,??????,??????????????,????????????????
???
25???? ??????????(Marssonia) ??
??????????????,????? ?????????
??????,???,????????,?????????
26?????? 1??????????????? ?????????,???2-8?,????
? 2?7???8???????????????????,????
27?????? 1??????? 2??????????,????
3?????,??????????? 4???????????? 5?????
200??????85???250???
28??????
- ?? ?????
- ?? ??
- ????????,????,????????
- ???????,????????
????,????????,????????,???????
???,???????????
29???? ????? ???
????????,?? ?????,??,????
????????,???? ??????,???
?????
30- ??????
- 1?????
- 2????????????????????
- 3??????,???????????
31- ??????
- 1???????
- 2???????????????
- 3???????????
- 4?????
- ???4?????1????
- ?????25???1000?1??????????
32????
??????? ?????????????????,??????????????????????,
?????????????????,????????,????????????????,??????
???????????????????????,?????,????????,?????
33????????????????,??????,????,?????,???????????,??
???,?????????????,??,???
34?????? 1?????????????????,??3-4???????????????
?????????,?????????????,????????????,??????????,??
???????,???30-60???,?????? 2??????????????????
???,????,???,?????? 3???????????????????
35?????? 1???????,?????????
2?????,?????,?????,????? 3?????
75?????????500-700???
36????????????????????????,????????,?????????,?????
??????????????