???????????????? ?????? (Griffith,1928) - PowerPoint PPT Presentation

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???????????????? ?????? (Griffith,1928)

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Title: ???????????????? ?????? (Griffith,1928)


1
2. ???????
  • ????????????
  • ??????????
  • ?????????
  • ??????????

2
??? ??? ??????? (?????? ??????????????????? ????
????? ???????)
3
???????????????? ?????? (Griffith,1928)
4
??? (Avery-MacLeod-McCarthy, 1944)
5
??? ???????? ???????????? ?????????? ?????
(?????-????, 1948)
6
?????????????????
7
?????????????? ???? ? ????? (Brenner, 1964)
8
?????????????? ???? ? ????? (Yanofsky, 1967)
9
?????
10
???????????? in vivo
11
???????????? ????????
12
?????????
  • recA ttgatactgtat---gagcatacagtataat
  • rpsL ttgacaccttttc--ggcatcgccctaaaat
  • aroF ttgaaaacttta---ctttatgtgttatcgt
  • lpdA tttaaaaattgtt--aacaattttgtaaaat
  • cyoA tttacagtaatgt--aaccttcccgtaaaat
  • uvrD ttggcatctctg---acctcgctgatataat
  • atpI ttgaaatcacgg---gggcgcaccgtataat
  • tonB ttgaatatgattgc-tatttgcatttaaaat
  • rpsU tttacaaagcagca-gcaattgcagtaaaat
  • cirA ttgataattgtta--tcgtttgcattatcgt
  • fur ttttcatttaggc--gtggcaattctataat
  • ampC ttgtcacgctga---ttggtgtcgttacaat
  • uvrB gtgatgaactgttt-ttttatccagtataat
  • argC ttgacacacct----ctggtcatgatagtat
  • rpmH ttgactccggagt--gtacaattattacaat
  • rplT ttaacgttttta---actttttaattagaat
  • gyrA tttacctcaaactgcgcggctgtgttataat

13
????????
14
?????? ?????????
  • carB ttatcgagttaattgagcagtaccgtaaaac
  • nagB tttactggctaaa--ccagaaaacttatttt
  • ilvG ttgccagcccacgg-tcggtcgacttactgt
  • cysK atgtcattattt---cccttctgtatataga
  • smp ttgcttctggca---acattaagtctcaaat
  • amn gtgacatacta----tcggatgtgcggtaat
  • ileR atgccagcagag---ttttttatgcgttaat
  • fabB ttgttcggcgtac--aagtgtacgctattgt
  • dnaN atatcaaagaag---atttttcaaatttaat
  • argC tcgataaatggcg--gtaatttgtttttcat
  • araB ctgacgcttttt---atcgcaactctctact
  • sulA ttgtcactggatgt-actgtacatccataca
  • trpR gttactgatccgc--acgtttatgatatgct
  • dnaN ttgcaggaaaaact-ggtcaccatcgacaat
  • cspA ttgcatcacccgc--caatgcgtggcttaat
  • rpsU tcgcccctggaga--aagcctcgtgtatact
  • rpoH ttcacaagcttgcattgaacttgtggataaa

15
Lac - ????????
gcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaat
gcagctggca cgacaggtttcccgactggaaagcgggcagtgagcgcaa
cgcaattaatgtgagttagct cactcattaggcaccccaggctttaca
ctttatgcttccggctcgtatgttgtgtggaat tgtgagcggataacaa
tttcacacaggaaacagctatgaccatgattacgaattcgagct cggta
cccggggatcctctagagtcgacctgcaggcatgcaagcttggcactggc
cgtcg ttttacaacgtcgtgactgggaaaaccctggcgttacccaactt
aatcgccttgcagcac atccccctttcgccagctggcgtaatagcgaag
aggcccgcaccgatcgcccttcccaac agttgcgcagcctgaatggcga
atggcgcctgatgcggtattttctccttacgcatctgt

16
Lac - ????????
cgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaa
tgtgagttagct cactcattaggcaccccaggctttacactttatgct
tccggctcgtatgttgtgtggaat tgtgagcggataacaatttcacaca
ggaaacagctatgaccatgattacgaattcgagct

17
Lac - ????????
cgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaa
tgtgagttagct cactcattaggcaccccaggctttacactttatgct
tccggctcgtatgttgtgtggAat tgtgagcggataacaatttcacaca
ggaaacagctatgaccatgattacgaattcgagct

18
Lac - ????????
cgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaa
tgtgagttagct cactcattaggcaccccaggcTTtACActttatgct
tccggctcgTATgtTgtgtggAat tgtgagcggataacaatttcacaca
ggaaacagctatgaccatgattacgaattcgagct

19
???????????? - ????
20
???????????? ???
  • ??????????
  • ?????????????????
  • ???????????

21
???????????? ???? (????, 1961)
22
???????????? ???
23
????????
24
?????????-????-????????? ? ????
25
????????? ??????????
26
????? ????????? ??????????
dnaN ACATTATCCGTTAGGAGGATAAAAATG gyrA
GTGATACTTCAGGGAGGTTTTTTAATG serS
TCAATAAAAAAAGGAGTGTTTCGCATG bofA
CAAGCGAAGGAGATGAGAAGATTCATG csfB
GCTAACTGTACGGAGGTGGAGAAGATG xpaC
ATAGACACAGGAGTCGATTATCTCATG metS
ACATTCTGATTAGGAGGTTTCAAGATG gcaD
AAAAGGGATATTGGAGGCCAATAAATG spoVC
TATGTGACTAAGGGAGGATTCGCCATG ftsH
GCTTACTGTGGGAGGAGGTAAGGAATG pabB
AAAGAAAATAGAGGAATGATACAAATG rplJ
CAAGAATCTACAGGAGGTGTAACCATG tufA
AAAGCTCTTAAGGAGGATTTTAGAATG rpsJ
TGTAGGCGAAAAGGAGGGAAAATAATG rpoA
CGTTTTGAAGGAGGGTTTTAAGTAATG rplM
AGATCATTTAGGAGGGGAAATTCAATG
27
????? ????????? ??????????
dnaN ACATTATCCGTTAGGAGGATAAAAATG gyrA
GTGATACTTCAGGGAGGTTTTTTAATG serS
TCAATAAAAAAAGGAGTGTTTCGCATG bofA
CAAGCGAAGGAGATGAGAAGATTCATG csfB
GCTAACTGTACGGAGGTGGAGAAGATG xpaC
ATAGACACAGGAGTCGATTATCTCATG metS
ACATTCTGATTAGGAGGTTTCAAGATG gcaD
AAAAGGGATATTGGAGGCCAATAAATG spoVC
TATGTGACTAAGGGAGGATTCGCCATG ftsH
GCTTACTGTGGGAGGAGGTAAGGAATG pabB
AAAGAAAATAGAGGAATGATACAAATG rplJ
CAAGAATCTACAGGAGGTGTAACCATG tufA
AAAGCTCTTAAGGAGGATTTTAGAATG rpsJ
TGTAGGCGAAAAGGAGGGAAAATAATG rpoA
CGTTTTGAAGGAGGGTTTTAAGTAATG rplM
AGATCATTTAGGAGGGGAAATTCAATG
28
????????? ??????????
29
???? ????????? ??????????
30
?????????? ??????????
31
????????
32
????????
33
?????????? - ????
34
??????????(?????????, ???????????,
?????????????????)
35
??? ????????????
??????? (7500 ??)
17 ??????? (2500 ??)
36
????????? (????, ???????, 1975)
37
???????? ??????????
38
?????????????? ?????????
39
?????????? ???
40
?????????? ??? (?????????-?????, 1958)
41
????????? ?????????? ???
42
????????? ??????????? ?????? ???
43
???-?????????? III
44
???-?????????? III
45
???????? ?????????? ????????? ????????
46
?????????????? ?????????
47
??????????? ??????????????? ???
48
?????????? ??? ? ?????
49
???????? ??????????
50
?????????? - ????
51
3. ?????????(? ????????? ?????)
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