Wavelength and Light Properties

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Wavelength and Light Properties
(l)
High Energy
Low Energy
n c / l E hn
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Discovery of the Fluorescence 1852
Sir George Gabriel Stokes (1819-1903)
Fruorite
Fluorescence
- escence - means "to become" in Latin. - Fluor
- comes from the Fluorite (fluorspar)
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Fluorescence
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Spectral Shift Filters ( Stokes Shift)
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Fluorochrome (khroma crwma color) a
fluorescent molecule Fluorophore
(-phore joroz to bear, carrying) a
component of a molecule which causes a molecule
to be fluorescent Fluorescent dye XXX is
labeled with a fluorescent dye Fluorescent
probe XXX is the Fluorescent probe for ...
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Fluorochromes
Fluorescein isothiocyanate (FITC)
Hoechst
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Fluorochromes
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Fluorochromes
Quantum Yield
total integrated photon emission over the entire
spectral band of the fluorophore
S1 gt S2
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Fluorochromes
FITC ME - 93 000 QY - 0,87
Phycoerythrin (PE) ME - 1 960 000 QY - 0,82
Drs E.D. Wieder and L. Gibson, Gladstone Inst.
Of Virology Immunology, San-Francisco, CA
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Fluorescent probes for DNA
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Immunolabeling
Ag Primary antibody (Ab )-FITC
Ag AC Secondary Antibody ( Ab )-FITC
Ag AC -Avidin (a) Biotin (b)-FITC
Ag AC Protein A (pA)-FITC
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Fluorescent Proteins
Ag Antibody-Fluorochome
Protein Fluorochome (Fluorescent Fusion Protein)
Green Fluorescent Protein
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Fluorescent probes for Metal Ions
Fluo-3
Ca
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Fluorochromes
Phycoerythrin (phycoerithrobilin)
Texas Red
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Conjugated Fluorochromes
Red 613
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Conjugated Fluorochromes
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Quantum dot
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Fluorochromes Cytometer
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Cells (Beads etc)
Fluorochromes
Staining
Instrumentation the art and science of
measurement
Data Analysis Presentation
Sorting
Examples