Wang haitao

1
Wang haitao March 14 2011
2

• Loss of one type of sensory input can cause
  improved functionality of other sensory systems.
  Whereas this form of plasticity, cross-modal
  plasticity, is well established, the molecular
  and cellular mechanisms underlying it are still
  unclear.
• blind individuals compensate their lack of visual
  input by responding to somatosensory or auditory
  input with improved sensitivity and accuracy. The
  brain can adapt to sensory deprivation in one
  modality (e.g., visual cortex) by increasing
  plasticity and retuning neuronal circuits in
  other remaining modalities.

3
(No Transcript)
4
Rectification index I (-60 mV)/ I(40mV)

• GluR2-containing receptors
• GluR2-lacking receptors

5
Methods

• In vitro whole cell recording
• Infection of Neocortical Neurons In Vivo
• In Vivo Microdialysis
• In Vivo Extracellular Recording
• Biochemical Analysis
• Immunohistochemistry

6
VD Drives GluR1 into Layer 4 to Layer 2/3
Synapses of the Barrel Cortex in Juvenile Rats
GFP-GluR1
7
VD Drives GluR1 into Layer 4 to Layer 2/3
Synapses of the Barrel Cortex in Juvenile Rats
8
VD Procedure for 2 Days Occludes LTP in the
Barrel Cortex
9
VD did not alter properties of NMDA component and
inhibitory component
10
VD Procedure for 2 Days Occludes LTP in the
Barrel Cortex
11
Serotonin Mediates VD-Driven Synaptic GluR1
Delivery in the Juvenile Rat Barrel Cortex
12
Serotonin Mediates VD-Driven Synaptic GluR1
Delivery in the Juvenile Rat Barrel Cortex
13
Serotonin Facilitates Synaptic GluR1 Delivery
14
Serotonin Facilitates Synaptic GluR1 Delivery
15
Serotonin Facilitates Synaptic GluR1 Deliveryc
16
VD Induces Phosphorylation of GluR1 at a Site
Critical for Its Synaptic Delivery
17
VD Sharpens Functional Whisker-Barrel Map
18

• Visual deprivation (VD) drives AMPA receptors
  into synapses in the rat barrel cortex
• Seoronin mediates VD-induced facilitation of
  synaptic AMPA receptor delivery
• VD increases activation of ERK and increase of
  phosphorylation of GluR1 at Ser845
• VD sharpens the map in the barrel cortex

19
(No Transcript)
20

• Thanks