Supplemental Figure S1

1
Supplemental Figure S1
Immunoblot analysis. RPE cells were incubated in
serum free DMEM (25 mM glucose) in the presence
or absence of 10 µM PF 4708671 for 42-44 hours
and then were stimulated with 1 µg/ml insulin
and lysed.
2
Rap
IR
o/n
chronic
- - - - -
insulin
HIF1a
actin
DMEM
MEM
Supplemental Figure S2
Immunoblot analysis. RPE cells were maintained
either in DMEM (25 mM glucose) or in MEM (5.5 mM
glucose). Cells were incubated in serum-free
respective medium for 42-44 hr and then
stimulated with 1 µg/ml insulin for 15 min and
lysed, Rap 10 nM rapamycin o/n cells were
pretreated with rapamycin overnight before
stimulation chronic cells pre-treated with
rapamycin for 2 weeks before stimulation IR
cells were pre-treated with 1 µg/ml insulin
overnight before stimulation.