Objectives

1
Objectives

• E Describe the different processes used in
  cloning
• C Interpret data on the cloning process
• A Evaluate the moral and social implications
  taking into account humanitarian and
  anti-globalisation ideas

2
Methods

• Making Gene fragments
• Conversion of mRNA to cDNA using reverse
  transcriptase
• Cutting DNA at palindromic recognition sequences
  using restriction endonucleases
• PCRCloning
• In vitro
• In vivo
• Benefits and disadvantages Production of
  transgenic organisms