Prodigiosin Synthesis

1
Prodigiosin Synthesis

• UV-induced Colorless Mutants of Serratia
  marcescens

2
The Normal Pathway to Prodigiosin
alanine
MAP(g)
enzyme x
amino acids
Prodigiosin
enzyme z
MBC(ws)
proline
enzyme y
3
Serratia marcescens D1 (wild type) (DNA is not
mutated for any of the genes)
ALA
MAP(g)
enzyme x
amino acids
Prodigiosin
enzyme z
PRO
MBC(ws)
enzyme y
4
Serratia marcescens D1 (wild type) (DNA is not
mutated for any of the genes)
MAP(g)
ala
enzyme x
amino acids
Prodigiosin
enzyme z
MBC(ws)
pro
enzyme y
5
Serratia marcescens D1 (wild type) (DNA is not
mutated for any of the genes)
ala
MAP(g)
enzyme x
Prodigiosin
amino acids
enzyme z
MBC(ws)
pro
enzyme y
6
Serratia marcescens ( ) (DNA is mutated in
enzyme x gene)
ala
MAP(g)
enzyme x
amino acids
Prodigiosin
enzyme z
MBC(ws)
pro
enzyme y
7
Serratia marcescens ( ) (DNA is mutated in
enzyme x gene)
ALA
MAP(g)
enzyme x
amino acids
Prodigiosin
enzyme z
PRO
MBC(ws)
enzyme y
8
Serratia marcescens ( ) (DNA is mutated in
enzyme x gene)
ALA
MAP(g)
enzyme x
amino acids
Prodigiosin
enzyme z
MBC(ws)
pro
enzyme y
9
Serratia marcescens ( ) (DNA is mutated in
enzyme x gene)
ALA
MAP(g)
enzyme x
amino acids
Prodigiosin
enzyme z
MBC(ws)
pro
enzyme y
ala
MAP(g)
enzyme x
amino acids
Prodigiosin
enzyme z
MBC(ws)
pro
enzyme y
Serratia marcescens ( ) (DNA is mutated in
enzyme y gene)
10
Serratia marcescens ( ) (DNA is mutated in
enzyme x gene)
ALA
MAP(g)
enzyme x
amino acids
Prodigiosin
enzyme z
MBC(ws)
pro
enzyme y
ALA
MAP(g)
enzyme x
amino acids
Prodigiosin
enzyme z
PRO
MBC(ws)
enzyme y
Serratia marcescens ( ) (DNA is mutated in
enzyme y gene)
11
Serratia marcescens ( ) (DNA is mutated in
enzyme x gene)
ALA
MAP(g)
enzyme x
amino acids
Prodigiosin
enzyme z
MBC(ws)
pro
enzyme y
MAP(g)
ala
enzyme x
amino acids
Prodigiosin
enzyme z
PRO
MBC(ws)
enzyme y
Serratia marcescens ( ) (DNA is mutated in
enzyme y gene)
12
Serratia marcescens ( ) (DNA is mutated in
enzyme x gene)
Serratia marcescens ( ) (DNA is mutated in
enzyme x gene)
ALA
MAP(g)
enzyme x
amino acids
Prodigiosin
enzyme z
MBC(ws)
pro
933
WCF
enzyme y
MAP(g)
ala
enzyme x
amino acids
Prodigiosin
enzyme z
PRO
MBC(ws)
enzyme y
Serratia marcescens ( ) (DNA is mutated in
enzyme y gene)
13
Serratia marcescens ( ) (DNA is mutated in
enzyme x gene)
933
MAP(g)
ALA
MAP(g)
enzyme x
Prodigiosin
amino acids
Prodigiosin
enzyme z
MBC(ws)
pro
933
WCF
enzyme y
MAP(g)
MAP(g)
ala
enzyme x
amino acids
Prodigiosin
enzyme z
PRO
MBC(ws)
enzyme y
Serratia marcescens ( ) (DNA is mutated in
enzyme y gene)
WCF
14
Serratia marcescens ( ) (DNA is mutated in
enzyme x gene)
933
ALA
MAP(g)
enzyme x
amino acids
Prodigiosin
enzyme z
MBC(ws)
pro
933
WCF
enzyme y
MBC(ws)
MAP(g)
ala
enzyme x
Prodigiosin
amino acids
Prodigiosin
enzyme z
MBC(ws)
PRO
MBC(ws)
enzyme y
Serratia marcescens ( ) (DNA is mutated in
enzyme y gene)
WCF
15
Serratia marcescens D1 (wild type) (DNA is not
mutated for any of the genes)
ala
MAP(g)
enzyme x
Prodigiosin
amino acids
enzyme z
MBC(ws)
pro
enzyme y
Why is D1 (so capable!) such a poor donor to
either mutant
D1
933
D1
WCF
MAP(g)
MBC(ws)
compared to cross-feeding from either mutant?