Mix concrete

1
Mix concrete
Transform bacteria with genes

• Develop genes
• bonding agent
• - quorum sensing
• - linear dna clock
• - kill switch
• - damp-proofing

Develop concrete disks
Incubate transformed bacteria in nutrient broth
Mould concrete into testing disks
Create control nutrient broth with
non-transformed bacteria
Grow to application density
Create microcracks
Create control microcrack
(Activate linear DNA clock)
Develop surface-tension reducing agent
Record position and size of microcracks
Model linear dna clock
Apply bacteria to external cracks on concrete /
microinjection
Develop microinjection
Model optimal density quorum sensing
Quorum sensing
Model optimal density time linear clock
Bacteria swim down crack / away from oxygen
gradient
Model bacterial migration
(Linear DNA clock says when)
Secrete spider silk while swimming
When?
Bacteria reproduce to optimal density
Develop secretion mechanism
Secrete bonding agent (glue, CaCO3)
Go
Allow bond to dry / set
Model crack-filling / lattice formation
Cell death/lysis / kill switch
Go
Test whether crack has been filled

• Develop testing procedure
• compressive strength
• tensile strength
• - ultrasound
• -SEM

Test strength of control and repaired and
unmodified concrete / crack
Create data about microcrack size/position vs.
concrete strength in unmodified concrete over
time. Also data about concrete that hasnt cracked