Name:_______________

1
Quiz 8 Biol 203 Fall 2013
The Arristaless-like (Arl) gene below carries a
homeodomain DNA binding domain (HD). Furthermore,
it has a proven activator domain (A) and
repressor domain (R). Possible WT mRNAs with
A-HD, which have activator-like properties as a
protein, promote cell growth Possible WT mRNAs
with R-HD, which have repressor-like properties
as a protein, inhibit cell growth. mRNAs with HD
alone never occur. WT mRNAs with A-R-HD code for
non-functional proteins.
Name_______________
Lab_______________
20pts
Arl


HD

R
A
E1
E2
E3
1
2




Express TxFactor E1
Express TxFactors E1, E2
RBP binding sites (where RBP would bind in the
transcribed RNA)
Express TxFactors E1, E2, E3
Q1(15pts).
If RBP typeB suppresses 3 acceptor site usage in
the unspliced RNA at position 2, then mark cells
below where A-HD mRNAs will be made.
If RBP typeA suppresses 3 acceptor site usage in
the unspliced RNA at position 1, then mark cells
below where R-HD mRNAs will be made.
E1 and E2 are both needed to drive RBP typeA
expression E1, E2, and E3 (all3) needed to drive
RBP typeB expression E1, E2, and E3 (all3) needed
to drive Arl expression
30 cells in WT Antennal Tissue
A null allele within a RBP gene will give the
same phenotype within the 30 cells as a null
allele for Arl. Which one?______
Q2(5pts).
Using a flow chart and a few words please
describe how first strand cDNA is derived from an
mRNA? What does the first 5 nucleotide of
this first strand cDNA correspond to in the
mRNA?__________________ What does the last
3 nucleotide of this first strand cDNA
correspond to in the mRNA? __________________
What does the first 5 nucleotide of this first
strand cDNA correspond to in the gDNA?
__________________ What does the last 3
nucleotide of this first strand cDNA correspond
to in the gDNA? __________________
2
Quiz 8 Biol 203 Fall 2013
The Arristaless-like (Arl) gene below carries a
homeodomain DNA binding domain (HD). Furthermore,
it has a proven activator domain (A) and
repressor domain (R). Possible WT mRNAs with
A-HD, which have activator-like properties as a
protein, promote cell growth Possible WT mRNAs
with R-HD, which have repressor-like properties
as a protein, inhibit cell growth. mRNAs with HD
alone never occur. WT mRNAs with A-R-HD code for
non-functional proteins.
Name_______________
Lab_______________
20pts
Arl


HD

R
A
E1
E2
E3
1
2




Express TxFactor E1
Express TxFactors E1, E2
RBP binding sites (where RBP would bind in the
transcribed RNA)
Express TxFactors E1, E2, E3
Q1(15pts).
If RBP typeA suppresses 3 acceptor site usage in
the unspliced RNA at position 1, then mark cells
below where R-HD mRNAs will be made.
If RBP typeB suppresses 3 acceptor site usage in
the unspliced RNA at position 2, then mark cells
below where A-HD mRNAs will be made.
E1 and E2 are both needed to drive RBP typeA
expression E1, E2, and E3 (all3) needed to drive
RBP typeB expression E1, E2, and E3 (all3) needed
to drive Arl expression
30 cells in WT Antennal Tissue
A null allele within a RBP gene will give the
same phenotype within the 30 cells as a null
allele for Arl. Which one?______
Q2(5pts).
Using a flow chart and a few words please
describe how first strand cDNA is derived from an
mRNA? What does the first 5 nucleotide of
this first strand cDNA correspond to in the
gDNA?__________________ What does the last
3 nucleotide of this first strand cDNA
correspond to in the gDNA? __________________
What does the first 5 nucleotide of this first
strand cDNA correspond to in the mRNA?
__________________ What does the last 3
nucleotide of this first strand cDNA correspond
to in the mRNA? __________________