Semi

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Semi Rigid Isolator Units

• Charles River Laboratories
• Consulting Staffing Services

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Terminology

• Flexible Film
• SRI
• HEPA
• Port
• Gasket
• Magnahelic Gauge

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Isolator History Function

• Used since 1950s
• Flexible Film or Rigid contruction
• Biocontainment
• Negative Air Pressure
• Bioexclusion
• Positive Air Pressure
• Biosecurity at Charles River

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Features

• Typical forward facing port
• Gasket will change color when sealed properly

Hinge
Gasket
Clamp
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Features
Flexible Front
HEPA Filter
External Filter
Glove Arm
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Isolator Supplies
O-Rings
Nitrile Gloves
Electrical Tape
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Leak Detection

• Snoop
• Apply liquid to area suspected of leak and watch
  for bubbles
• Alternative
• Mild Soap solution

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Air flow
Positive
Negative
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Keeping it Clean

• Wescodyne
• Clidox
• Bleach Solution
• Windex/Cinch

• Peracetic Acid
• Chemical Incompatibility
• Check MSDS

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Ergonomics

• Your back should always be as straight as
  possible.
• Hydraulic tables allow the user to adjust the
  height!

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Filter Change

• Filters should be checked daily
• Filters need to be changed when the magnahelic
  gauge increases by 0.05 (i.e. from 1.0 to 1.5)

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Filter Pop Quiz

• Which filter assembly needs to be replaced?

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Testing for deterioration

• This rim should appear green even when stretched.
• If small holes or cracks are present glove must
  be replaced.
• Punctures from animal bites or instruments also
  warrant glove replacement.

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Semi-Rigid Isolator Daily Checks and Inspections

• Glove and flexible surface checks
• Check the magnahelic gauge
• Pull the gloves inside out, pulling on the cuff
  only
• Check for holes, punctures, tears, or stretched
  areas
• Then recheck the magnahelic for proper pressure
• Wipe down the outside of the flexible front
  surface

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Magnahelic and Ball Valve
Magnahelic Gauge
Ball Valve
Fan Motor
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Semi-Rigid Isolator Daily Checks and
Inspections

• Magnehelic gauge check
• Check gauge and record twice daily
• Gauge reads what the pressure is inside the
  isolator (.10 or -.10)
• Reads in Inches of Water
• Not reading proper pressure report immediately
• DO NOT adjust equipment unless properly trained

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Filter Locations
HEPA filter
External Filter
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But how do you use it?
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Introduction of supplies
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Supplies always in unit

• Disinfectants
• Sharps
• Forceps
• Scraper
• Marking Pens
• Absorbent Towels
• Paper Bags
• Hand Broom

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Supplies introduced to unit

• Feed
• Bedding
• Cages
• Water
• Sterilized vs Irradiated

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Port Door
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How to Use the Semi-Rigid Isolator Units

• Loading the Isolator
• Remove all jewelry, watches and especially rings
• Don protective glove liners
• Gather appropriate supplies
• Open the outside port door
• Place supplies into port Spray down supplies
  with disinfectant/sterilent
• Allow sufficient contact time
• 20 40 minutes
• Secure outer door

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How to Use the Semi-Rigid Isolator Units

• Loading the Isolator continued
• Push the sleeves into the isolator by holding
  onto cuff
• Over stretching the gloves will cause damage
• Open the port door
• Remove items from port into the isolator
• After all items have been removed, shut and lock
  port door clamps

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How to Use the Semi-Rigid Isolator Units

• Unloading the Isolator
• Once everything is ready to be removed from the
  isolator spray the inside of the port completely
• Spray all items before placing them into port
• Once all items are sprayed down with Clidox the
  port can be loaded
• After everything is loaded the port is then
  closed
• Set the timer according to SOP/Chemical
  Manufacturer

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How to Use the Semi-Rigid Isolator Units

• Unloading the Isolator
• When the timer goes off you may unload the port
• Contaminated items should be disposed/sanitized
  appropriately.

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How to Use the Semi-Rigid Isolator Units

• Label door with time initials
• Set exposure timer
• Document event in log

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Introducing/Removing Animals to Isolator

• Supplies
• Biosafety Cabinet
• Plastic bottle/containers
• Nalgene
• Disinfectant
• Forceps

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Introducing/Removing Animals to Isolator

• Transfer into bottle/container inside BSC
• Leave lids unsealed until all containers are
  filled
• Use same introduction procedures as supplies
• Ensure animals are promptly removed from
  bottle/container
• Air supply in sealed container must be
  established

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Removing Animals from Isolator

• Return to plastic bottles/containers
• Double wrap in bag
• Paper or plastic? ?
• Use regular spray out procedures
• Air supply for animals limited!

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Semi-Rigid Isolator Daily Checks and
Inspections

• Inside of the isolator check
• Shelving and all surfaces for cracks or cuts in
  the plastic
• Light bulb and casing
• Port and clamps to ensure that it seals inside
  and out
• If any one of these items are wrong or not
  working report immediately
• DO NOT leave trash inside the isolator
• DO NOT leave the isolator dirty
• Animal checks inside the isolators
• Check feed and water levels in each cage
• Observe the animals to ensure health status
• Check for and change any wet cages

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Semi-Rigid Isolator weekly Checks and Inspections

• Cage changing
• Before cage changing begins ensure that all of
  the daily checks have been completed
• Make sure all items for changing are inside the
  isolator before starting, this will help with
  being efficient
• Change cages
• After the cages have been changed remove all
  trash and dirty bedding from the isolator
• Make sure all animals are present and safely in
  their home cages
• Wash down the inside of the isolator, all
  surfaces including the inside of the glove
  sleeves
• Once all trash is removed wipe down the port
• Filter checks
• Check the outside filter for any dirt, debris, or
  damage
• Report to a supervisor if something is found

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