Title PI name/institution

1
Integrated Enzyme-Logic systems for Monitoring
and Treating Autonomously Injured Soldiers
Joseph Wang (UCSD) and Evgeny Katz (Clarkson
University)
Project Objectives
To develop next-generation sense and treat
autonomous devices for enhancing the survival
rate among injured soldiers in the battlefield.
Technical Approach
Accomplishments/Impact/Transitions

• Extend enzyme logic gate functionality towards
  relevant injuries in undiluted human serum
  samples with high fidelity
• Developed enzymatic filters to enhance
  separation in readouts of different input
  combinations.
• First demonstration of the applicability of
  enzyme gates in a porcine model of blunt liver
  injury
• First demonstration of minimally-invasive
  microneedle sensor arrays for monitoring
  fluctuations in key metabolites within the
  transdermal fluid
• Developed wearable textile-based electronic
  transducers, µ-electronic backbone to control
  logic gates
• Developed signal-responsive materials and
  interfaces actuated by enzyme-based injury
  related gates for targeted drug delivery and
  sustained drug release formulation
• Impact Development of autonomous body-worn
  devices /systems for minimally-invasive biomarker
  sampling
• Transitioning towards Pharmacy-on-Demand
  paradigm for the controlled release of a
  therapeutic intervention in response to signals
  generated by enzyme logic sensor

• Our approach involves the design, optimization,
  and evaluation of novel biochemical cascades
  composed of concatenated enzyme-based logic gates
  capable of performing Boolean logic operations on
  biomarker inputs.
• This system will be applied to the autonomous
  processing of physiological information and
  interfaced with electronic transducers
  signal-responsive drug-delivery materials /
  actuators.
• By using multiple key injury biomarkers as
  inputs for the enzyme gates, the biochemical
  logic system provides high-fidelity diagnostics
  when compared with single biomarker sensors.