Where are bacteriophages found? - PowerPoint PPT Presentation

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Where are bacteriophages found?

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Lytic life cycle Screening of a DNA Library Filamentous phage life cycle Where are bacteriophages found? ... Integration of lambda DNA into the host. – PowerPoint PPT presentation

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Title: Where are bacteriophages found?


1
Where are bacteriophages found?
Source Phages counted Soil 104/gm River 103
- 109/ml Seawater 107/ml Feces -
human 109/gm
Estimated of phages in biosphere 1030
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Lytic life cycle
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Bacteriophage vectors are useful for cloning
larger DNA fragments
Bacteriophage Vectors
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X
X
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In vitro packaging Uso di batteri lisogeni
contenenti Differenti mutazioni nei geni
strutturali I batteri sono cresciuti a densità
voluta E poi indotti al ciclo litico
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Screening of a DNA Library
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Genoteca Insieme di segmenti di DNA o cDNA
inseriti in vettori fagici o plasmidici
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Filamentous phages
RF Replicative Form
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Filamentous phage life cycle
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