Lysozyme

1
Lysozyme
http//en.wikipedia.org/wiki/Lysozyme

• A.k.a
• Muramidase OR
• N-acetylmuramide glycanhydrolase

2
Lysozyme

• An enzyme found naturally in
• egg white
• human tears
• saliva (as well as other body fluids)
• Capable of destroying the cell walls of certain
  bacteria.
• Acts as a mild antiseptic.

3
Amino Sequence
Fig 5. Lysozyme amino acid sequence alignment for
five organisms (Dmelanogaste-fly, EcoligsnAA-E.
coli, GgallusgsnAA-chicken, HsapiensgsnA-human,
and Mmusculusgsn-mouse). Number to the left and
right of the sequences and above each sequence
group indicate amino acid number. Letters
represent amino acids, with dashes (-) inserted
to maximize sequence alignment. Black boxes
indicate amino acid conservation.
4
Structure
Made with 117 amino acid residues and has a
heterogeneous sequence.
1REM
133L
1IWV
Quaternary Structure (4)

• lthttp//www.rcsb.org/pdb/cgi/explore.cgi?pdbId1re
  mgt

5
Function

• Lysozyme attacks peptidoglycans and hydrolyzes
  the glycosidic bond that connects N-acetylmuramic
  acid with the fourth carbon atom of
  N-acetylglucosamine. It does this by binding to
  the peptidoglycan molecule in the binding site
  within the prominent cleft between its two
  domains.
• Peptidoglycans- sugars amino acids that form a
  mesh-like layer outside the plasma membrane of
  bacteria, forming the cell wall.
• Hydrolysis- Decomposition of a chemical compound
  by reaction with water, such as the dissociation
  of a dissolved salt or the catalytic conversion
  of starch to glucose.
• http//en.wikipedia.org/wiki/Lysozyme

6
Present in other Organisms

• bacteriophages (viruses that infect bacteria)
• bacteria
• plants
• animals

7
Misc

• Lysozyme was first identified and named by
  Alexander Fleming, who recognized its
  bacteriolytic properties. (1922)
• Bottlefed babies are 3 times likely to have
  diarrheal disease, because they lack lysozyme in
  their diet... its in breast milk! Lysozyme is a
  natural form of protection from pathogens.
• Bacteriolysis -destruction or dissolution of
  bacterial cells

8
Works Cited

• Yikrazuul. "Lysozyme." Wikipedia. N.p., 14 Oct.
  2009. Web. 14 Oct. 2009. lthttp//en.wikipedia.org/
  wiki/Lysozymegt.
• Muraki, M., et al. "HUMAN LYSOZYME WITH
  MAN-B1,4-GLCNAC COVALENTLY ATTACHED TO ASP53."
  RCSB Protein Data Bank. N.p., 14 Oct. 2009.
  Web. 14 Oct. 2009. lthttp//www.rcsb.org/pdb/cgi/
  explore.cgi?pdbId1remgt.
• 3. Secretlens. "Drool." DeviantArt. N.p., 15 July
  2009. Web. 14 Oct. 2009. lthttp//secretlens.devi
  antart.com/art/Drool-129635147gt.
• 4. funkipunk . Tears Speak." DeviantArt. N.p.,
  13 Nov. 2007. Web. 14 Oct. 2009.
  lthttp//funkipunk.deviantart.com/art/Tears-Speak
  -69773894gt.
• 5. Caothicart . Dark Pikachu." DeviantArt. N.p.,
  10 June 2007. Web. 14 Oct. 2009.
  lthttp//caothicart.deviantart.com/art/Dark-Pikac
  hu-57318376gt.
• Chueh, Luke . I ASKED FOR SCRAMBLED. LukeChueh
  N.p., 2003. Web. 14 Oct. 2009. lthttp//www.lukec
  hueh.com/paintings/i-asked-for-scrambled.htmlgt.
• Stryer L. 1995. Biochemistry. 4th ed. New York
  W.H. Freeman and Company. p 372.
• "MacDNAsis Analysis of Lysozyme Genbank search
  results." Davidson College. N.p., 30 Nov. 2001.
  Web. 14 Oct. 2009. lthttp//www.bio.davidson.edu/C
  ourses/Molbio/MolStudents/01grnoland/
  MacDNAsis.htmlgt.