Sections to Skip for Ch 2

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Sections to Skip for Ch 2
Figure 2.3 - Enzyme digestion of Abs Monoclonal
Antibodies Fig 2.12 2.13
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Examples of Problematic Ab binding to various
structures
Pocket Penicillin - antibiotic that IgE can bind
to and initiate inflammatory response Groove DNA
- Systemic Lupus Erythematosus (Lupus) Extended
Surface Lysozyme - Hen egg component
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Questions From Class
Is IgM only pentameric? IgM is made as a monomer
in the cell and then assembled in the cell into a
pentamer, with the help of the J chain. After
assembly, it is secreted from the cell as a
pentamer. Therefore, the secreted form of IgM is
only present as a pentamer
Why does a T helper cell (CD4 TH1 have to
activate a macrophage after the same macrophage
has already phagocytosed a pathogen? Macrophages
have two main effector functions (see Fig 1.14)
1) phagocytosis and 2) effector signaling by
secretion of cytokines. TH1 cells will
themselves secrete cytokines to increase the
overall effector activity of the macrophage
including the secretion of cytokines from the
same macrophage (Fig 1.27, 3.11 and 8.15).
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Questions From Class (continued)
What does soma mean with respect to somatic
recombination? Soma refers to all the cells of
the body (soma is latin for body) except germ
cells. Germline configuration refers to the
arrangement of genes in all of the somatic cells
and germ cells that do not undergo somatic
recombination. In cells that have the unique
capability to undergo somatic recombination, such
as B and T cells, germline configuration refers
to gene arrangement before those cells have
undergone gene rearrangement (by the process of
somatic recombination)
A) Can the two types of light chain(? and ?) be
made at the same time in the same B cell? B) How
does that affect Ab diversity? A) No. One B cell
only expresses one type of light chain. B)
Please see the following slide for the correct
calculation.
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Random Recombination of Gene segments is one
factor contributing to diversity

10,530 3,369,600 Ig molecules
120)
(200
X
Only one light chain loci gives rise to one
functional polypeptide!