??af??e?a 1

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? ? F ? ? ? ? ? 23 ? ???µ?s? t?? d?µ?? t??
???µat????
Genes VIII - ??ad?µa???? ??d?se?? 2004
2
?????a 23.1 S?µf??a µe t? µ??t??? ?s????p?a?, ?
?at????? ? ??? µ?a? ??s?? p??sdes?? st? DNA
e?a?t?ta? ap? t? s?????t??s? t?? p??te???? p??
p??sd??eta? s a?t??.
Genes VIII - ??ad?µa???? ??d?se?? 2004
3
?????a 23.2 ??? se ??a? ?p?????t? s??µat?st???
?????e?s?µata, ?? µeta??af???? pa?????te? ?a? ?
RNA p???µe??s? de? µp????? ?a p??sde???? s
a?t??. ??t??eta, e?? ?? µeta??af???? pa?????te?
?a? ? RNA p???µe??s? p??sde???? p??t?? st??
?p?????t? ?a? ?a??e??s??? ??a sta?e?? s?µp????
??a????, t?te ? p??sdes? t?? ?st???? de? e??a?
d??at?.
Genes VIII - ??ad?µa???? ??d?se?? 2004
4
?????a 23.3 ?? d??aµ??? µ??t??? ??a t? µeta??af?
t?? ???µat???? p??ß??pe? t? d??s? pa?a???t?? ??
?p???? ???s?µ?p????? t?? e????e?a p?? pa???eta?
ap? t?? ?d????s? t?? ATP ??a ?a e?t?p?s??? ta
?????e?s?µata ap? s???e???µ??e? pe?????? t?? DNA.
Genes VIII - ??ad?µa???? ??d?se?? 2004
5
Genes VIII - ??ad?µa???? ??d?se?? 2004
?????a 23.4 ?a s?µp???a a?ad?aµ??f?s?? µp????? ?a
p?????s??? t?? ???s??s? t?? ?????e?s?µ?t?? ?at?
µ???? t?? DNA, ?a e?t?p?s??? ????????t??? ta
?????e?s?µata ap? t? DNA ? ?a a??????? t??
ap?stas? d?ad?????? ?????e?s?µ?t??.
6
Genes VIII - ??ad?µa???? ??d?se?? 2004
?????a 23.5 ?a s?µp???a a?ad?aµ??f?s?? µp????? ?a
ta????µ????? s?µf??a µe t?? ?p?µ???da ATP?s?? p??
pe???aµß?????.
7
?????a 23.6 ??a s?µp???? a?ad?aµ??f?s??
p??sd??eta? st? ???µat??? µ?s? e??? e?e???p???t?
? e??? ?atast???a.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.7 ? ??µ?????? ?p?d???a? HR ?a? ?
pa?????ta? NF1 de? µp????? ?a p??sde????
ta?t?????a st?? ?p?????t? MMTV a? a?t?? ??e?
µ??f? ??aµµ???? DNA, a??? µp????? ?a p??sde????
a? t? DNA ß??s?eta? st?? ep?f??e?a e???
?????e?s?µat??.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.8 ?? ?-te????? ????? t?? ?st???? H3 ?a?
H4 µp????? ?a a?et????????, ?a µe????????? ? ?a
f?sf?????????? se a??et?? ??se??.
Genes VIII - ??ad?µa???? ??d?se?? 2004
10
?????a 23.9 ? a?et????s? t?? ?st???? H3 ?a? H4
s?et??eta? µe t?? e?e??? e?? ? µe?????s? µe t??
a?e?e??? ?at?stas? t?? ???µat????.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.10 ?? pe??ss?te?e? ??se?? t??p?p???s??
t?? ?st???? ?f?sta?ta? ??a µ??ad???, e?d??? t?p?
t??p?p???s??. Ost?s?, µe????? ???e? ??se?? µp??e?
?a ap?te???? st????? pe??ss?te??? t?p??
t??p?p???s??. ???sµ??e? t??p?p???se?? µp??e? ?a
s?et????ta? µe s???e???µ??e? ?e?t?????e? t??
???µat????.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.11 St? f?s? t?? a?t???af??, ?? ?st??e?
?f?sta?ta? a?et????s? p??? e?s?µat????? se
?????e?s?µata.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.12 ? a?et????s? p?? s?et??eta? µe t??
e?e???p???s? t?? ????d?a??? ??f?as??
p?a?µat?p??e?ta? µe ?µes? t??p?p???s? t?? ?st????
p?? s?????t??? ta ?????e?s?µata.
Genes VIII - ??ad?µa???? ??d?se?? 2004
14
?????a 23.13 ?? s??e?e???p???t?? µp??e? ?a
d?a??t??? e?e???t?te? HAT, ?? ?p??e? a?et????????
t?? ????? t?? ?????e?s?µ???? ?st????.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.14 ?a s?µp???a p?? t??p?p????? t? d?µ?
t?? ???µat???? ? t?? e?e???t?t? t?? f?????
?p?µ???de? st??e?s?? p?? ?a???????? t?? ??se??
d??s?? t??? p??? st? DNA, e???µ???? ?p?µ???de?
HAT ? HDAC p?? a?et???????? ? apa?et???????? t??
?st??e?, ?a??? ?a? ???e? ?p?µ???de? p?? as????
ep?p???? ep?d??se?? st? ???µat??? ? st? DNA.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.15 ??a s?µp???? ?atast???? ap?te?e?ta?
ap? t??a µ??? µ?a ?p?µ???da p??sdes?? st? DNA,
??a s???atast???a ?a? µ?a apa?et???s? t?? ?st????.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.16 ? a?et????s? t?? ?st???? e?e???p??e?
t? ???µat???, e?? ? µe?????s? t?? DNA ?a? t??
?st???? ape?e???p??e? t? ???µat???.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.17 ? e?e???p???s? e??? ?p?????t?
pe???aµß??e? a????? t?? p??sdes? e??? e?d????
e?e???p???t? ?a? st? s????e?a t? st?at?????s? ?a?
t? d??s? e??? s?µp????? a?ad?aµ??f?s?? ?a? e???
s?µp????? a?et????s?? t?? ???µat????.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.18 ?a p???ta????? ???µ?s?µata
µeta??a?µ???? µ???? ?? ?p??e? ste????ta? ????s??
JIL-1 ????? a??µa?? µ??f?????a ?a? e??a?
s?µp????µ??a ?a? ??? e?tetaµ??a. ? f?t???af?a
e??a? e??e???? p??sf??? t?? Kirsten M. Johansen.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.19 ?? fa???µe?? t?? p??????t?ta? ????
??s?? st? ???µa t?? µat??? t?? µ??a? p????pte?
?ta? t? ????d?? white e?s?µat??eta? ???t? se
ete?????µat???. ?a ??tta?a sta ?p??a t? white
e??a? ape?e???p???µ??? s??µat????? ?e????
pe?????? st? µ?t?, e?? ta ??tta?a sta ?p??a t?
white pa?aµ??e? e?e??? s??µat????? ??????e?
pe??????. ? ??tas? t?? fa???µ???? ?a?????eta? ap?
t? p?s? ???t? st?? ete?????µat??? ß??s?eta? t?
e?s?µat?µ??? ????d??. ? f?t???af?a e??a? e??e????
p??sf??? t?? Steve Henikoff.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.20 ? e??p??s? t?? ete?????µat????
ape?e???p??e? ta pa?a?e?µe?a ????d?a. ?
p??a??t?ta ?a ape?e???p????e? ??a ????d??
e?a?t?ta? ap? t?? ap?stas? p?? t? ?????e? ap? t??
p????a p???µe??sµ?? t?? ete?????µat????.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.21 ? e?e???t?ta t?? ???µat????
ep??e??eta? ap? d?af??et???? t??p?p???se?? t??
????? t?? H3.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.22 ? SUV39H1 e??a? µ?a
µe????t?a?sfe??s? t?? ?st???? p?? ep?d?? st? 9Lys
t?? ?st???? H3. ?????????, ? HP1 p??sd??eta? st?
µe?????µ??? a?t? ?st???.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.23 ? µe?????s? t?? ?st???? H3
d?µ?????e? µ?a ??s? p??sdes?? ??a t?? HP1.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.24 ? p??sdes? t?? HP1 st? µe?????µ???
?st??? H3 ap?te?e? t? ??a?sµa ??a t?? ap?s??p?s?
t?? ???µat????, epe?d? ep?p???? µ???a HP1
p??sd????ta? d?ad????? t? ??a d?p?a st? ???? p???
st? d??ta?? t?? ?????e?s?µ?t??.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.25 ? s??µat?sµ?? t?? ete?????µat????
a????e? ?ta? ? RAP1 p??sd??eta? st? DNA. ??
SIR3/SIR4 p??sd????ta? st? RAP1, ?a??? ?a? st??
?st??e? H3/H4. ?? s?µp???? p???µe???eta? ?at?
µ???? t?? ???µat???? ?a? e??a? d??at?? ?a s??d?e?
ta te??µe?? µe t? p??????? p???µa.
Genes VIII - ??ad?µa???? ??d?se?? 2004
27
?????a 23.26 ?? p??te??e? t?? ?µ?da? Pc-G de?
p???a???? t?? ?atast???, a??? e??a? ?pe????e? ??a
t? d?at???s? t??.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.27 ??af??et???? ???a??sµ??
???s?µ?p????? d?af??et????? µ??a??sµ???
a?t?st??µ?s?? t?? ????d?a??? d?s??, p???e?µ????
?a e??s??e? ? ??f?as? t?? ???µ?s?µat?? X µeta??
a?se????? ?a? ??????? at?µ??.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.28 ? f???s??det? p??????µ??f?a
p????pte? ???? t??a?a? ape?e???p???s??
d?af??et???? ???µ?s?µat?? ? se ???e p????????
??tta??. ??tta?a sta ?p??a t? e?e??? ???µ?s?µa ?
f??e? t? a?????µ??f? ????? fa???t?p? ??????
t?p??, e?? ??tta?a sta ?p??a t? e?e??? ???µ?s?µa
? f??e? t? a?????µ??f? - e?d??????? t?
µeta??a?µ??? fa???t?p?.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.29 ? µ??a??sµ?? ape?e???p???s?? t?? ?
ßas??eta? st? sta?e??p???s? t?? RNA t?? Xist, t?
?p??? ?a??pte? t? a?e?e??? ???µ?s?µa.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.30 ??a p??te??? SMC ap?te?e? µ?a
µ???da ????s?? (walker module) p?? f??e?
µ?t?ßa d?sµe?s?? ATP ?a? p??sdes?? st? DNA se
???e ???? t??, s??dedeµ??a µe spe???µ??a
spe???µata p?? e?????ta? µ?s? µ?a? pe??????
?????s??.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.31 ?? d?? ß?a????e? µ?a? ???te?s????
a?ad?p?????ta? se ????a 6. ?? ???es??e? ?????
p?? a????t? ste?e?d?aµ??f?s?, µe ????a 86
a??µesa st??? d?? ß?a?????? t???.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.32 ?? p??te??e? SMC d?µe?????ta? µe
a?t?pa??????e? a????ep?d??se?? t?? ?e?t?????
spe???µ???? spe??aµ?t?? t???. ?a? ta d?? ???a
???e ?p?µ???da? ????? µ?t?ßa d?sµe?s?? ATP ?a?
p??sdes?? st? DNA. ?? ???es??e? µp????? ?a
s??µat????? µ?a e?tetaµ??? d?µ? p?? ep?t??pe? t?
s??des? d?? d?af??et???? µ????? DNA.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.33 ?? ???te?s??e? µp????? ?a ????????
st?? ?????s? t???, p???a???ta? ?ts? t? s?µp????s?
t?? DNA.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.34 ?? ???es??e? µp????? ?a d?µe??st???
µe e?d?µ???a??? a????ep?d??se?? ?a? ?a
s??µat?s??? st? s????e?a p???µe?? p?? s??d???ta?
t?s? sta ???a t?? ?p?µ???d?? ?s? ?a? st?? ?????s?
t???. ??a t?t??a d?µ? ?a µp????se ?a s????ate?
d?? µ???a DNA pe??ß?????t?? ta.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.35 ?? ???te?s??e? e?t?p????ta? se
???????? t? µ???? e??? µ?t?t???? ???µ?s?µat??. ??
DNA e??a? ???µat?sµ??? ??????? e?? ?? ???te?s??e?
??t???e?. ? f?t???af?a e??a? e??e???? p??sf???
t?? Ana Losada ?a? t?? Tatsuya Hirano.
Genes VIII - ??ad?µa???? ??d?se?? 2004
37
?????a 23.36 ?? p??t?p? t?? µe?????s?? µp??e? ?a
d?a????ste? ap? ??a ????µ? p?? a?a??????e? ??
?p?st??µa µ??? t?? ?µ?µe?????µ??e? ??se??.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.37 ?? p??t?p? µe?????s?? e????eta? ap?
t?e?? t?p??? e???µ??, ap? t??? ?p????? ?????
?a?a?t???ste? ? de novo µe????t?a?sfe??s? ?a? ?
µe????t?a?sfe??s? d?at???s??, e?? ?? ap?µe????se?
de? ????? ta?t?p????e? a??µ?.
Genes VIII - ??ad?µa???? ??d?se?? 2004
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?????a 23.38 ?? DNA µp??e? ?a ap?µe??????e?
afa????ta? t? µe????µ?da, t? µe?????µ??? ß?s? ?
???????? t? µe?????µ??? ?????e?t?d??. ? afa??es?
t?? ß?s?? ? t?? ?????e?t?d??? apa?te? t??
a?t??at?stas? t??? ap? ??a s?st?µa ep?d?????s??.
Genes VIII - ??ad?µa???? ??d?se?? 2004
40
?????a 23.39 ?? t?p??? p??t?p? e?t?p?µat??
p??ß??pe? ?t? ??a µe?????µ??? a?????µ??f? e??a?
a?e?e???. ??? t? a?????µ??f? a?t? e??a? µ?t?????
p????e?s??, t?te pa?aµ??e? e?e??? µ??? t? pat????
a?????µ??f?, t? ?p??? ?a??stata?, ?ts?,
apa?a?t?t? ??a t?? ep?ß??s?. ?at? t? s??µat?sµ?
t?? ?aµet??, t? p??t?p? µe?????s?? epa????eta?
st?? a????? t?? ?at?stas?, ?ts? ?ste ??a ta
spe?µat???tta?a ?a f????? t?? pat???? t?p? ?a?
??a ta ????tta?a t? µ?t????.
Genes VIII - ??ad?µa???? ??d?se?? 2004
41
?????a 23.40 ? pe????? ICR e??a? µe?????µ??? st?
pat???? a?????µ??f?, ?p?? t? Igf2 e??a? e?e???
?a? t? H19 a?e?e???. ? ICR e??a? µ? µe?????µ???
st? µ?t???? a?????µ??f?, ?p?? t? Igf2 e??a?
a?e?e??? ?a? t? H19 e?e???.
Genes VIII - ??ad?µa???? ??d?se?? 2004
42
?????a 23.41 ? µ? µe?????µ??? pe????? ICR
p??sd??e? t?? p??te??? CTCF ?a? d?a ?? µ???t??
p?? pa?eµp?d??e? ??a? pa?a?e?µe?? e??s??t? ?a
e?e???p???se? t? Igf2.
Genes VIII - ??ad?µa???? ??d?se?? 2004
43
?????a 23.42 ?e ????????µe t? a???ß?? s?µßa??e?
sta p??te????? s?µp???a t?? ???µat???? ?at? t?
d????e?a t?? a?t???af??.
Genes VIII - ??ad?µa???? ??d?se?? 2004
44
?????a 23.43 ?? a?et????µ???? p????e? t??
?????e?s?µ?t?? s??t?????ta? ?a? d?a??µ??ta?
t??a?a sta ???d?a t?? ???at????? ???µat???? ?at?
t?? a?t???af?. ???e ???at???? ???d?? ap?te?e?ta?
ap? ??a µe??µa pa?a??? (a?et????µ????) ?a?
?a???????? (µ? a?et????µ????) ?????e?s?µ????
p??????.
Genes VIII - ??ad?µa???? ??d?se?? 2004
45
?????a 23.44 ? µ??f? t?? p??te???? Sup35
?a?????e? t? d??at?t?ta te?µat?sµ?? t??
µet?f?as??.
Genes VIII - ??ad?µa???? ??d?se?? 2004
46
?????a 23.45 ? ?e?s??t???µe?? p??te??? Sup35
µetat??peta? st? µ??f? PSI, ef?s?? ?p???e?
µ?sa st? ??tta?? ? a??µa?? a?t? µ??f? t??
p??te????.
Genes VIII - ??ad?µa???? ??d?se?? 2004
47
?????a 23.46 ?a?a?? p??te??? µe µ??f? PSI
µp??e? ?a µetat???e? t? fa???t?p? t?? ??µ?? ap?
psi- se PSI.
Genes VIII - ??ad?µa???? ??d?se?? 2004
48
?????a 23.47 ? p??te??? PrPSc µp??e? ?a µ????e?
??a ??? µ??? e?? a?t? f??e? e?d??e?? p??te???
PrPC t?? ?d??? t?p??.
Genes VIII - ??ad?µa???? ??d?se?? 2004