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Možnosti evropského výzkumu v oblasti související
s fytosanitární problematikou
Jana Hajšlová Ústav chemie a analýzy potravin,
VŠCHT Praha Jana.hajslova_at_vscht.cz Národní
fytosanitární workshop 3.3.2009, Praha
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Activity 2.1

Sustainable production and management of
biological resources from land, forest and
aquatic environment
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KBBE-2010-1-2-06 Deepened and enlarged
cooperation between phytosanitary (statutory
plant health) research programmes - ERA-NET

• The aim is to prevent the introduction into the
  community of organisms harmful to plants or plant
  products or their spread within the Community.
  Due to climate changes and globalization, new
  threats are continuously emerging and the number
  and complexity of plant health problems increases
  every year, requiring enhanced research efforts.

Funding scheme Coordination and Support Action
(supporting action). The requested European
Community contribution shall not exceed EUR 1 000
000.
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KBBE-2010-1-2-05 Integrated pest management in
farming systems of major importance for Europe

• better targeted use of all available pest
  control measures, including preventive measures,
  observation and pest monitoring, pesticides and
  biocontrol agents,
• modelling and experimentation, including
  physiological and molecular understanding of
  plant-pathogen interactions and development of
  symptoms
• targeting farming systems in which reduction of
  pesticide use and better control of pests and
  pathogens is expected to have major effects at
  the European scale

Funding scheme Collaborative Project (large
scale integrating project). The requested
European Community contribution shall not exceed
EUR 9 000 000.
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Jaká jsou rizika pro konzumenty? Je lepší volit
bioprodukty?
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OLIVE OIL
? Samples from Czech market
23 extra virgin olive oils
from
organic farming (O1 O23) 23 extra virgin olive
oils

from conventional farming (C1 C23)
EXTRA VIRGIN OLIVE OIL The oil obtained from the
fruit of the olive tree solely by mechanical or
other physical means under the conditions.
particularly thermal conditions, that do not lead
to alternations in the oil, and which has not
undergone any treatment other than washing,
decantation, centrifugation and filtration.
? Method of analysis GCx GC/TOF MS
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PAHs in olive oil
Benzo(a)pyrene)
Sum of 16 PAH Sum of BaA, Chr, CPcdP,
5-MeChr, BcFln, BbF, BkF, BjF, BaP,
DBahA, BghiP, IcdP, DBahP, DBalP,
DBaiP, DBaeP
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PESTICIDE RESIDUES in olive oils
Chlorpyrifos-methyl Imazalil Iprodione Isofenpho
s Kresoxim methyl Lindane Malathion Metalaxyl Meth
acrifos Methamidophos Methidathion Mevinphos Monoc
rophos DDD DDE DDT Omethoate Oxyfluorfen Oxychlord
ane Paraoxon ethyl Parathion Parathion
methyl Penconazole Permethrin Phenothrin Phenthoa
te
Phosalone Phoshamidone Phosmet Pirimicarb Pirimip
hos methyl Procymidone Propham Pyridaben Quinalpho
s Sulfotep Tebuconazole Tetraconazole Tetradifon T
hiobendazol Tolclofos-methyl Tolylfluanid Triadim
efon Triazamate Triazophos Trifloxystrobin Trichlo
rfon Vinclozolin
Acephate Acrinathrin Aldrin Azinfos ethyl Azinfos
methyl Beta cyfluthrin Bifenthrin Bromophos
ethyl Bromophos methyl Brompropylate Bupirimate Ca
ptan Carbaryl Carbophenothion Cyhalothrin Cypermet
hrin Cyprodinyl Deltamethrin Diazinon Dieldrin Dif
enoconazole Difenylamin Dichlofluanid Dichlorvos
Dimethoate Endosulfan alfa
Endosulfan beta Endosulfan sulfat Endrin Ethion Et
rimfos Fenamiphos Fenarimol Fenchlorphos Fenitroth
ion Fenoxycarb Fenthion Fenvalerate
Fludioxonil Folpet Formothion HCB Heptachlor Hept
enophos Hexythiazox HCH alfa HCH beta HCH
delta Chlorfenviphos Chloropham Chlorothalonil Ch
lorpyrifos
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PESTICIDES DETECTED IN OLIVE OILS
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Pesticides
RESIDUE CONVENTIONAL CONVENTIONAL CONVENTIONAL CONVENTIONAL ORGANIC ORGANIC ORGANIC ORGANIC
RESIDUE n mean (µg/kg) Min - Max (µg/kg) of positive samples n mean (µg/kg) Min - Max (µg/kg) of positive samples
Endosulfan sulphate 22 55.2 26.9 - 210.2 96 8 33.8 24.5 - 43.5 35
Fenthion 8 127.1 58.0 - 319 35 0 - - 0
Chlorpyrifos 3 40.5 37.8 - 41.5 13 2 31.7   9
n - number of positive samples
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Rezidua pesticidu ve vzorcích z ekologického a
konvencního zemedelství na ceském trhu
komodita produkcní systém pocet detekovaných reziduí
mrkev organické 0
mrkev organické 0
mrkev organické 0
mrkev konvencní 2
mrkev konvencní 4
mrkev konvencní 1
jablka organické 0
jablka organické 0
jablka organické 0
jablka konvencní 6
jablka konvencní 3
jablka konvencní 6
komodita produkcní systém pocet detekovaných reziduí
rajcata organické 0
rajcata organické 1
rajcata organické 0
rajcata konvencní 5
rajcata konvencní 11
rajcata konvencní 9
papriky organické 0
papriky organické 1
papriky konvencní 3
papriky konvencní 5
papriky konvencní 3
papriky konvencní 9
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282 pesticides in LC- MS/MS multiresidue method
Column Supelco Discovery C18 , Mobile phase
MeOH (A), 0.01M amm. acetate (B) Flow 0.3 ml/min
Kovalczuk T., Lacina O., Jech M., Poustka J.,
Hajšlová J. Novel approach to fast determination
of multiple pesticide residues using UPLC-MS/MS.
Food Addit. Cont. 25(4), 444457 (2008).
PERFORMANCE OF METHOD EMPLOYING QQQ

for all analytes 4 identification points at
concentration levels ? 0.01 mg/kg available?
requirements of 2002/657/EC met
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Various contaminants may occur in cereal grains

• PESTICIDES
• MYCOTOXINS
• PLANT TOXINS
• POPs
• PAHs

Is it possible to examine the presence of these
toxins in a single run?
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Pesticides and all regulated mycotoxins
within
single run in 8 minutes (UPLC- TOF MS)
Pesticides
Mycotoxins
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NEW
AMBIENT MASS SPECTROMETRY
opens the doors to many challenging applications
in various areas including food / feed control
- Reduced / minimal sample prep - No
chromatographic separation
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Imazalil in citrus fruit detected in 1 minute
Cl pattern
direct screening of peel
Element composition
Concentration measured in crude extract by DART
TOF MS was 34 µg/kg.
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