LC/MS/MS??????????????

1
LC/MS/MS??????????????

• ????????? ?? ?

2
????
????????????????????????
??????????????????????????
?????????????????????????
???????????????????????? ????????????????????
???? ??????????????????????(MBC)?
?????????????????????????????????????????????ESI??
??LC/MS/MS?????ng/L????????? ???????????
3
LC/MS?LC/MS/MS?????
4
MS/MS????
???????????????
??(?????) ????- SIM?? ????????-Full
Scan?????
???(????????????) ??????????????????
?????????(???) ???????????????(MS/MS)???
??????????????????????? ??????(S/N???)???????
(????????????????)
5
MS/MS ???
Single Stage Mass Spectrum
MS/MS Mass Spectrum
Isolation Event
Collisional
Activation Event
6
??????????MS/MS
1. Inject
3. Fragment
2. Isolate
4. Detect
7
Collision Energy???
MBC Standard 2ppm
0.18ppm
0.017ppm
8
MBC??(2ppm)?????
MH
Full Scan Mass Spectra
MS/MS Spectra
9
???????????
Full Scan m/z192
Relative Abundance
m/z
MS/MS m/z160
Relative Abundance
Relative Abundance
Time (min)
m/z
10
??????????
Full Scan m/z192
Relative Abundance
Relative Abundance
m/z
MS/MS m/z160
Relative Abundance
Time (min)
m/z
11
???
??(ppm)
12
MS/MS??
1. ?????? 1/10
2. ??BG?? BG 1/100
????S/N????
13
30
297
20
m/z 355
m/z 355
25
40
297
m/z 355
297
MS/MS ?? (Br-NP-1EC)
14
??? ?????
1.??? ??? ??? ???? ??????? ??????
2.???? ??? ???? ??? COD????? ????5 ??
15
MBC
ESI MS/MS 192 gt50-200
2900?
MBC
ESI MS/MS 192 gt160
2500?
ESI MS/MS 192 gt50-200
RT6.46?
MBC
??? ??? ???
16
A
MBC
ESI MS/MS 192 gt50-200
2600?
MBC
2300?
ESI MS/MS 192 gt160
ESI MS/MS 192 gt50-200
RT5.25?
A
RT6.46?
MBC
???????????
17
??? ??????
1. ??? ??? ??? ????
2. ???? ??? ???? ???
3. ??? ??? ???
4. ??? ??? ???
18
MBC
A
ESI MS/MS 192 gt50-200
MBC
ESI MS/MS 192 gt160
ESI MS/MS 192 gt50-200
A
MBC
??? ??? ???
19
MBC
A
ESI MS/MS 192 gt50-200
MBC
ESI MS/MS 192 gt160
ESI MS/MS 192 gt50-200
A
MBC
???????????
20
B
MBC
A
ESI MS/MS 192 gt50-200
MBC
ESI MS/MS 192 gt160
ESI MS/MS 192 gt50-200
A
B
MBC
??? ??? ???
21
A
MBC
ESI MS/MS 192 gt50-200
MBC
ESI MS/MS 192 gt160
ESI MS/MS 192 gt50-200
A
MBC
??? ??? ???
22
MBC?MS/MS????????????-????????????
23
???????????????
???????????????????? ???????MS????????????? ??????
?
???????MS??????? ms-l_at_ee-net.ne.jp
???????? dioxin-l_at_ee-net.ne.jp
24
Web???????????
????????????????
????????????abstract?? http//www.ee-net.ne.jp/dio
xin2000/share.html
ltvolume, page no.gt 47, 9-12 ltEnglish
titlegt Relative Contribution of Chlorinated
Naphthalenes, Biphenyls, Dibenzofurans and
Dibenzo-p-Dioxins to Toxic Equivalents in Biota
from the South Coast of the Baltic sea.
ltJapanese titlegt ??????????PCN, PCB, PCDD,
PCDF????????????? ltauthorsgt J. Falandysz, K.
Kannan, M. Kawano and C. Rappe ltkey wordsgt TCDD
TEQs, Baltic sea , PCN, PCB, PCDD, PCDF,
sediment, plankton, mussel, crab, fish,
cormorant, wildlife, seafood ltJapanese key
wordsgt TCDD?????, ????, PCN, PCB, PCDD, PCDF, ??,
??????, ???, ??, ?, ?, ??? ?, ???? ltcaptionsgt Tabl
e l. TCDD Equivalents (H4IIE EROD or H4IIE-Luc)
of dioxin-like PCBs, PCNs, PCDFs and PCDDs in
varioussamples from the southern of the Baltic
Sea(pg/g lipid wt) Table l. ????????????????????PC
Bs?PCNs?PCDFs?PCDDs?TCDD????(H4IIE-EROD???H4IIE- ?
??????)(pg/g????) ltsummarygt ???????????????????PC
N, PCB, PCDD, PCDF??????????????????? ????????????
?????????, ??????, ???, ??, ?, ?, ????, ???

25
???
MS/MS???
???????????????????????S/N???? ???????????????????
???????????????
?????????????????????? ????????????(?????)????????
?????????
?????
????????Web???????
26
????
1) T.A.Roberts et al, Metabolic Pathways of
Agrochemicals,1113-1137