The balancing act. Redox poise and signalling

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The balancing act. Redox poise and
signalling http//plantcell.lu.se/balancing
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Acknowledgements
Lund University Plant Cell Biology-Plant
Biochemistry
http//plantcell.lu.se Thomas
Pfannschmidt (Now University of Jena,
Germany) Dalibor Stys (Now S. Bohemia University,
Czech Republic) Anders Nilsson (Now Bruker
Spectroscopy, Lund) Sylvan Dubé (Now Laval
University, Canada) Paul Davison (Now Sheffield
University, U.K.) Jens Forsberg Jörgen
Ström Magnus Rosenquist (Sommarin lab) Laure
Fraysse (Kjellbom lab) H. Louise Race (Now
Aylesbury, U.K.) Collaborators Torbjörn
Drakenberg, Sture Forsén (Lund Physical Chemistry
2) Gerhard Link (University of Bochum,
Germany) James A. Sullivan, John C. Gray
(University of Cambridge, U.K.) Cartoons Sarah
Allen
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The bucket
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The searchlight
Allen, J. F. (2001) EMBO Reports 2, 542-544
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Light 1
Light 2
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The balancing act1.

• Transients, enhancement, and redox poise of
  cyclic electron flow.
• Rates and activities of components.

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Myers, J. (1971) Annu. Rev.Plant Physiol. 22,
289-312
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Hill, R. (1965) Essays Biochem. 1, 121-151
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Park, R. B. and Sane, P.V. (1971) Annu. Rev.
Plant Physiol.22, 395-430
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Albertsson, P.-Å. (2001) Trends Plant Sci. 6,
349-354
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Pfannschmidt, T. et al. (1999) IUBMB Life 48,
271-276
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The balancing act2.

• Protein phosphorylation and state transitions.
  Post-translational modification of the structure
  and function of proteins you already have.

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Allen, J. F. (1992) BBA 1098, 275-225 . Adapted
from Allen, J.F. et al. (1981) Nature 291, 25-29
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Updates on state transitions

• Wollman, F.-A. (2001) EMBO J. 20, 3623-3630
• The kinase and the site of redox control
• Kruse, O. (2001) Naturwissenschaften 88, 284-289
• Structure and the PS II-LHC II supercomplex
• Haldrup, A. et al. (2001) Trends Plant Sci., 6,
  301-305
• Binding change, PS I and Psa-H

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Dubé, S., Davison, P.A. and Allen, J. F.
(unpublished)
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State 1
State 2
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State 1
State 2
Allen, J. F. and Forsberg, J. (2001) Trends Plant
Sci.6, 317-326
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Nilsson, A. et al. (1997) JBC 272, 18350-18357
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The balancing act3.

• Redox control of gene expression. Changing
  transcription and the stoichiometry of complexes.

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Allen, J. F. (1992) BBA 1098, 275-225
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Pfannschmidt, T. et al. (1999) IUBMB Life 48,
271-276
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Pfannschmidt, T. et al. (1999) Nature 397, 625-628
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The balancing act4.

• Redox signalling and evolution.
• Why chloroplasts (and mitochondria) have genomes

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Allen, J. F. (1993) J. Theor. Biol. 165, 609-631
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Localization of Arabidopsis BAB09274 His
Kinase-GFP fusion protein in tobacco leaf
epidermal cells
Unpublished data of Sullivan, J. A., Gray, J.C.
(Cambridge) Rosenquist, M., Forsberg, J., Allen,
J. F. (Lund)
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Protein ID Organism Type of two-component signalling element Predicted subcellular location Probability of location
ycf26 Porphyra purpurea Sensor histidine kinase Chloroplast 1 1
BAB09274 Arabidopsis thaliana Hybrid histidine kinase Chloroplast thylakoid membrane 0.922 0.922
AAD03576 Arabidopsis thaliana Hybrid histidine kinase Chloroplast thylakoid membrane 0.632 0.632
ycf27 Porphyra purpurea Response regulator Chloroplast 1 1
ycf29 Porphyra purpurea Response regulator Chloroplast 1 1
AAD15535 Arabidopsis thaliana Response regulator Mitochondrial matrix space 0.628 0.628
AAD55287 Arabidopsis thaliana Response regulator Chloroplast stroma 0.864 0.864
BAB20581 Zea mays Response regulator Chloroplast stroma 0.938 0.938
BAA85112 Zea mays Response regulator Chloroplast thylakoid membrane 0.656 0.656
BAA85113 Zea mays Response regulator Chloroplast stroma 0.600 0.600
Two-component sensors and response regulators
predicted to be in chloroplasts and mitochondria
(by P-sort)
Forsberg, J. et al. (2001) Biochem. Soc. Trans.
29, 403-407
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Race, H. L., Herrmann, R. G. and Martin, W.
(1999) Trends Genet. 15, 364-370
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The balancing act. Redox poise and signalling

• Transients, enhancement, and redox poise of
  cyclic electron flow.
• Protein phosphorylation and state transitions.
• Redox control of gene expression.
• Redox signalling and evolution.

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Light 1
Light 2
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The balancing act. Redox poise and
signalling http//plantcell.lu.se/balancing