(TM)

1

??????? ?????????? ?????. ???????????? ?????.
12 ?????? 2011 ?. ????????? ???????????? ?
??????????????, 2 ????, ???????? ???????
2
???????? ??????
???????????? ????????????
??????????
??? ??????????, ????? ????? ??? ?????????? ??????
????????? ???????
3
????????????
?????????? ?????
????????????
??????????????
??????? ? ???????????? ????????? ??????
??????????????????? ??????
??????????????
??????????????
??????????????? ????? (TM)
4
??????? 1 ?????-???????
?????????? ????? i4 -gt i
5
???????
BACTERIORHODOPSIN
ATP SYNTHASE
????????? a-??????? ??? ????????? ???????? (gt 90
of TM ??????)
6
??????? 2 Beta-barrel
7
???????
PDB 2POR, PORINE
PDB 1EK9, from ESCHERICHIA COLI
Beta barrels ??????? ???????? ?????????????????
????????, ? ????? ???????? ??????????? ?
????????????
8
????? ????????? TM ??????
9
??????? von Heijne the positive-inside rule
10
????? ?????????????
11
http//www.tcdb.org/
Transporter Classification Database
12
V.W.X.Y.Z
13
????????? ????????????? TC

•  V (????? ) ????? ????????????
•  W (?????) - ???????? ??? ????????
  ????????????? ?????????? ???????? ???????
•  X (?????) - ????????? ?????? (??????
  ??????????????, ?.?. ???????? ???????????????????
  ?????????????, ?? ?????????? ????? ?????)
•  Y (?????) - ????????????  
• Z - ?????????? ????????????? ????????????????
  ???????

14
?????? ????? MALF_ECOLI, TC 3.A.1.1.1

• ???????? ?????????
• ?????????? ???
• Superfamily The ATP-binding Cassette (ABC)
• ?????????????? ????????

15
http//opm.phar.umich.edu/
?????????? ????? ???????????? ????????????
??????? ?Gtransfer, ? ?????? ??????????
d, z0, T ? f  ? ??????? ?????????, ? ??????? ???
Z ????????? ? ???????? ??????
16
? ??? ?????????
?? 15 ?? 30 ?? ?????? ????? ?????? ????? ?
???? ?? ????? ??????????????? ????????
????? ?72244 ???????? ????????. ?????????? - 279
(0.39 ), 2011
17
??????? ???????????? ??????????????? ????????

• ?? ??????? ?????????????
• C ?????? TMHMM (???????????? TM a-????????)
• ??? ß-???????? ????????? ?????? (????????,
  PRED-TMBB)
• ?? 3D ????????? ????????
• ?? ?????????????? ????????????

18
??? ??????? ???????? ???????? ??? ???????? ???
??? ??????? ???????? ?????????????
19
??????

• ?????? ??????? ???? (FP) ??????????? ???????
  ????????, ? ??? ????????????? ????????????????.
• ?????? ??????? ???? (FN) ??????????? ???????
  ????????, ? ??? ?? ?????.
• ?????? ???????? ???? ??????????? ??????????
  ??????? ???????? ?? ?????? ???????? ???????????.
  

20
????????? ?????????? ???????????? ROC-??????