????DNA?????????

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????DNA?????????

• ????
• ??????????????
• ?????

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????
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DNA ?????
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DNA???
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DNA???(PRIMASE)

• ?DNA?????RNA????,?????2??????????????????????????
  ??????????

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DNA???
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DNA?????
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??????(SSB)
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???(nucleases)

• ????????????????,??DNase ? RNase??,???????????????
  ????????????????????3-5??????????????RNaseH?5-
  3??????

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DNA???

• DNA???????DNA?????????????????????5??????????????
  ??3?????????????????????ATP?DNA???????DNA????????
  ?,?????4??????,DNA???I?????????????

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?????????MCM2-7????????????????????

• ??MCM2-7????????????DNA??????????????????(ORC)?CD
  c6?Cdt1????DNA ??,??G1-S?????CDc45??????CDc7?Cdk2?
  ?????,?????,???MCM???????ORC???????????????,??????
  ??(XENOPUS)???????,????????DNA???????????????????,
  ?DNA???80bp?,ORC?MCM???11?DNA?????,MCM???????,??
  MCM????????CDc45?DNA???MCM??????DNA??????,

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??(?)

• ???CDc7??????????,?????Cdk2 ???CDc45??????????????
  ??,DNA?????????????????????????MCM2-7????

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???DNA???????????
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?????????????????G1????????
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?????DNA????????????????
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ORC?MCM???????82-bp???DNA???
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MCM????DNA????,?ORC?DNA????
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??MCM???????????
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???DNA??CDC45?DNA?????????
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MCM????????CDC7???
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CDC45?????????????D???
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DNA??????
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??

• ??MCM????????82bp?DNA??,ORC??????80bp?DNA??,????G1
  ?MCM?????DNA???
• ??DNA??????82bp?,MCM??????????,?ORC????MCM???????
  ?ORC???DNA????
• ??MCM????DNA????????CDC6?CDT1?
• ???ORC?CDC6?CDT1???DNA?????MCM???
• ??MCM??????,ORC??????DNA??????ORC??MCM???????????

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??(?)

• ????MCM????CDC7??,MCM4?CDC7?????????????D??????,C
  DC45MCM??1,??????MCM??????CDC45?
• ??DNA??????????????,?????CDC45????????MCM?????????
  ??
• ??????,MCM???????????????,????????106?MCM????????
  ????MCM????G1???,?GI/S??????????