??????????????????????????,???????????????????,????????????????????????????????????????caspases?

1
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?????????????? ???
2
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• ??????????????,???????????????????????
  ????????????,?????????????????????????????????????
  ???????????,??????????????????????????????????????
  ??

3
???????

• ??????(?????)
• ??????????????????????????,???????????
  ????????,????????????????????????????????????????c
  aspases?
• ?????(?????)
• ???????????,????C??????????????,??????
  ??,??????caspase?
• Caspases?????????????(cystein aspartic
  cidspecific proteases) ?8? 9? ???, 3? 6?7????
• PS??????(phosphatidylserine)
• ??????????PS???Caspases????????????,??????????????
  ??

4
???????????? ????????(phosphatidylserine
,PS)??????? ???????????? ?????PS????
??
????(??????Annexin ??Annexin B1?)??PS?????
Blood,199789112132

5
?????????

• ???
• ??
• ??
• ??
• ???????
• ????

6
?????????

• ?????????????????????DNA??????????,???????????

7
(No Transcript)
8
?????????
9
??????????????
From Siemens and Dr. Wu Zhanhong
10
(No Transcript)
11
???PET/CT ??
?????
??
???
12
??? PET/CT ???????
13

?? ?? ??
????? ??????? ???? ?????,?????,???? ???????? ????,??? ???????? ???????? ??????? ?????????? ??????,?????? ???,???? ?????? ????????? ?????????? ?????? ???????????? ???? ?????? DNA ??????? ?????( ladder)?? ????,???,???????? DNA ??????? ??????, ????? ????, ???????? ?? ?????? ????? ???????? ?????? ????????????? ?????????? ????? ?????? ????,?????? ?????? ??,????? ?????? ????????? ????? ????????????

14
Annexin ????????

• ?????(PET??)
• ?11C??18F??64Cu??124I??94mTc?
• ?????(SPECT??)
• ?68?67 Ga??123I??125I??131I??
  111In??99mTc ?
• ????
• ????
• ?????????
• N2S2?HYNIC?MAG3? iminothiolane?
  EDDA? EC?MBP? tricarbony? SDH?BTAP?

15
???????????-99mTc-HYNIC-AnxV

• Apoptosis was induced in isolated human PMNs by
  incubation in 2 saline for 17 22 h at 37 ?
• Abscesses were induced in mice by
  intramuscular injection of bacteria or turpentine

17h ??? 22h ??? 4h ID/g 24h ID/g
??????? 71.60.01 74.90.02 ????? 1.250.09 3.750.83
???? 48.60.01 47.20.02 ?????? 1.020.16 0.720.17
Penn DL, et al.Apoptotic abscess imaging with
99mTc-HYNIC-rh-Annexin-V. Nucl Med Biol. 2010
Jan37(1)29-34.
16

• ????,??????
• ????????
• ????????
• ??
• ???????
• ?????

17
?????

• On postnatal day 7, rat pups in the experimental
  group were exposed to six injections of ketamine
  (20 mg/kg at 2-h intervals)?
• On postnatal day 35, 37 MBq of 18F-annexin V.
• The uptake of 18F-annexin V was significantly
  increased in the ROI of ketamine-treated rats.
  Additionally, the duration of annexin V tracer
  washout was prolonged

18
99mTc-Hynic-rh-Annexin V??????????? ??In vivo
imaging of apoptosis by 99mTc-Annexin V
scintigraphyvisual analysis in relation to
treatment response Kartachova M, Haas RLM, Olmos
RAV,et.al Radiotherapy and Oncology 72 (2004)
333339
19
?,60y,NHL?????????,???????? A ???,???????(???) B??
?1??,??????? C?D??????????99mTc-Hynic-rh-Annexin
V SPECT????,????????????????(???) E?F?SPECT/CT????
,????????????????
20
?,26y,???,??????????? A????MR,?????? B????3?MR,???
???(???) C?D?????????99mTc-Hynic-rh-Annexin V
SPECT????,???????????????(???)
E?F?SPECT/CT????,??????????????
21
?,47y,NHL,????????????,??????(22
Gy)?????? A????,?????????? B????1?,????????? C?
D??????????99mTc-Hynic-rh-Annexin V SPECT????
E?F?SPECT/CT????,???????????????
22
99mTc-Hynic-rh-Annexin V????????????? ??99mTc-HYN
IC-annexin V SPECT imaging of acute stroke and
its response to neuroprotective therapy with
anti-Fas ligand antibody Blankenberg FG, Kalinyak
J, Liu L,et.al European Journal of Nuclear
Medicine and Molecular Imaging Vol. 33, No. 5,
May 2006
23
???????2(3)?DWI (??)? 99mTc-HYNIC-annexin V
SPECT(??)??????????????????DWI?????????????
SPECT?????????????????,???????????
24
?????????3?(???)?1???(???)99mTc-HYNIC-annexin V
SPECT?????,????????3?????????,?????????
25
????????1(2)?DWI?99mTc-HYNIC-annexin V
SPECT??????DWI????????????????????????(???),??????
99mTc-HYNIC-annexin V ?????
26
Annexin B1

• ????????????,?????????????????????Annexin
  ??????????????99mTc??,??SPECT?????
• 99mTc-HYNIC-Annexin B1
• 99mTc -Annexin B1
• ??????????,??????????????????18F??Annexin
  B1??????

27
??????????
??????????????
?????
????
18F-SFB-Annexin B1
?Fas??????????(Annexin V????????)
??????????
??????
?????,PET/CT????(HE? TUNEL????)
28
18F-SFB- Annexin B1??(1)
11 MeV ?????
???(H218O) 18F??
(p,n)??
29
18F-SFB- Annexin B1??(2)

• ?????????
• ??18F-SFB
• QMA???18F????K222? K2CO3????????
• ??,??SFB????4-????????????????(DMSO)
• ?????,C18???,????????????????
• ??,??O-(N-?????)N,N,N,N-?????????(TSTU)
• ??,??,??,??,??

30
18F-SFB- Annexin B1??(3)

• 18F-SFB??Annexin B1??
• 18F-SFB????,??Annexin B1/BBS ?????????????????18F-
  SFB-Annexin B1

31
18F-SFB- Annexin B1
?????????? ???????????
32
18F-SFB- Annexin B1??
????????TUNEL?HE??
PET/CT
33
Acknowledgements

• ??? ????
• ??? ??
• ???????????????
• ??????????????
• ????????????????
• ????
• ????????????????????
• ???????????
• ????? ?

34
???? ????