Title: Immunology of CELIAC DISEASE
1Immunology of CELIAC DISEASE
- Ibrahim, Ibrahim
- Mentor Dr. Sanil Manavalan
- Columbia University, College of Physicians and
Surgeons
2- Celiac disease
- a digestive disease that damages the small
intestine and interferes with absorption of
nutrients from food - it is a genetic disorder
- some of the symptoms of celiac disease are
diarrhea, weight loss, malnutrition, etc - when people with celiac disease eat foods
containing gluten, their immune system responds
by damaging the small intestine - Diagnosis is by intestinal biopsy histological
grading and presence of serum auto- antibodies. - because the body's own immune system causes the
damage, celiac disease is considered an
autoimmune disorder
3- AUTOIMMUNE DISEASE condition in which the
immune system mistakenly attacks the body's own
organs and tissues - Type 1 Diabetes affects the pancreas
- Systemic Lupus erythmatotosus (Lupus) affects
the skin - Crohns Disease- affects the gut
- Multiple Sclerosis- affects the brain
4Key Terms
- Cytokines proteins secreted by macrophages and
leukocytes that stimulate or inhibit the
proliferation or function of immune cells. - Interleukin cytokines that only affect
leukocytes. - Lymphocyte A type of leukocyte (white blood
cell) of the immune system. - T-Regulatory Cell slows down and stops immune
response - T-Helper Cell has antigen-receptors, releases
cytokines - Natural Killer Cell kills macrophage and bad
stuff - Cytotoxic T-Cell kills cells that produce
foreign antigens such as cells infected by
viruses - B-Cell creates antibodies.
- Antigen invading microbe
- Antibody protein that fights bacteria and
viruses.
Lymphocytes
5The Gut Lumen
- Villi- (singular villus) are tiny, finger-like
structures that protrude from the wall of the
intestine - -Microvilli- hair-like structure on the surface
of absorptive and secretory epithelial cells - Epithelial cells - layer of cells which line the
GI tract
Diagram
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8GUT LUMEN
GUT LUMEN
Glaidin
Enterocytes
LAMINA PROPRIA AND INTRA EPITHILIAL SPACE
9Objective
- To find the cytokine levels in the serum of
patients in the following groups - Healthy Patients (Control)
- Active Celiac Disease Patients
- Celiac Disease patients on Gluten-free diets
- Refractory Disease Patients
- The following cytokines were checked in the serum
of these patients - IL-1ß
- IL-6
- IL-8
- IL-12 p70
- IFN-?
- TNF-a
10Methods
- Dilution of StandardsStandards were used to
calibrate the flow cytometer. The Standards were
diluted by 11, 12, 14, 18, 116, 132, 164,
1125, 1250, and 1500. The standards were just
FACS buffer solutions in water. - Calibration of CytometerTo do this, we had
solutions of the cytokines we were testing. After
this we calibrated the equipment so it recognizes
which wavelengths of light are representative of
specific cytokines. - Excel SpreadsheetTo analyze the results, and
present them to the scientific community, we used
Excel spreadsheet to put the data into a
comprehensible graph format.
11Flow Cytometry
DEFINITION technique for counting, examining and
sorting microscopic particles (such as cells)
suspended in a stream of fluid ROLE IN OUR
EXPERIMENT To analyze concentrations of
cytokines in the sera of patients using capture
beads which detect the cytokines.
12Hypothesis
- Increased serum levels of inflammatory cytokines
in patients with active and refractory celiac
disease - Normal levels of cytokines in gluten free diet
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14Results
15Results Cont.
16Conclusions
Pro-inflammatory cytokines such as Interferon
gamma and IL-8 seemed to be increased in both
patients with active disease and Celiac patients
on a gluten-free diet.
We are analyzing the data on an individual
patient by patient basis to determine if there is
a correlation with the biopsy grade for each
patient
17Acknowledgements
- Dr. Sanil Manavalan - Mentor
- Dr. Jamal Naiyer Co-mentor
- Sindhu Kunduvaram Undergraduate Student
- Dr. Sat Bhattacharya Coordinator OF HCS
- Mrs. Susan Farenholtz Coordinator of Project
Seed - Harlem Children Society
- Project Seed
18Cytokines
Cytokine IL-1ß IL-6 IL-8 IL-12 p70 IFN-? TNF-a Principal cell Targets and biological effects Endothelial cells activation B cells proliferation of antibody producing cells Activator of neutrophils NK cells and T cells IFN-gamma synthesis, increased cytolytic activity Macrophages Activation Endothelial cells Activation