Four Channels Data Acquisition System for Silicon Photomultipliers

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Four Channels Data Acquisition System for Silicon
Photomultipliers
Mateusz Baszczyk, Piotr Dorosz, Sebastian
Glab,Wojciech Kucewicz, Lukasz Mik, Maria Sapor
Department of Electronics, AGH University of
Science and Technology, Al. Mickiewicza 30,
30-059 Krakow, Poland e-mail kucewicz_at_agh.edu.pl s
glab_at_agh.edu.pl
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Agenda

• Fluorescence
• Silicon Photomultiplier description
• Description of previous measurement system
• Four channel data acquisition system
• Results
• Summary
• Future work.

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Why do we measure fluorescence light intensity?

• Dye-labelled cells can be count by fluorescence
  light intensity measurement
• At low concentrations the fluorescence intensity
  is generally proportional to the concentrations
  of fluorophore.

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Fluorescein and Bio-Tek filters spectra
fluorescein
resorufin
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Optical block
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Silicon Photomultiplier

• The idea appears in the late 90-ties
• Photon Detection with High Gain Avalanche
  Photodiode Arrays S. Vasile, P. Gothoskar, R.
  Farrell, D. Sdrulla
• Silicon avalanche photodiodes on the base of
  metal-resistor-semiconductor (MRS) structures V.
  Saveliev, V. Golovin

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Structure
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Current - Voltage Characteristic
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Photodiode Operation Mode
1 photon generates 1 electron-hole pair
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Avalanche Operation Mode
1 photon generates 10-200 electron-hole pairs
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Geiger Operation Mode
1 photon generates 105 - 106 electron-hole pairs
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Typical SiPM Parameters
sensL s1020 Hamamatsu S10362-11-025C
Spectral Range (?) 400-1100 nm 320-900 nm
Peak Spectral Response (?) 490 nm 440 nm
Breakdown Voltage (Vbr) 28.6 V ---
Operating Voltage (V) 30.6 V 70.82 V
Microcell Gain 106 2.75 105
SPM Pixel Active Area F 1 mm F 1 mm
Number of Pixels 400 1600
Dark Rate (MHz) 0.42 0.3
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Previous Measurement System For Fluorescence
Agilent 33250 generator
trigger signal
gate pulses
Picosecond 2600C generator
Cuvette holder with specimen
GPA amplifier
QDC device
PC with Labview application
excitation filter
emission filter
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First Measurement System For Fluorescence
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Acquisition System
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Acquisition System
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Front-end ASIC
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Front-end ASIC
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Front-end ASIC
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Linearity high gain
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Linearity low gain
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Measurement results
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Measurement results
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Measurement results
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Measurement results
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Measurement results
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Measurement results
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Light Intensity
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Light Intensity
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Light Intensity
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Without Coincidence
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Coincidence
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Results of fluorescence light measurements
y -572500 x² 223.6 x 6458000
fluorescein y -373800 x² 170.3 x 14030000
resorufin
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Influence of Temperature and Bias
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Dark Current Rate
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Influence of Temperature and Bias
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Conclusions

• We have designed, built and optimized readout
  system for detection of single photons
• We have tested readout circuit with light and
  confirmed that it is linear
• We can distinguish a dozen or so photons and
  eliminate thermal generation from measurement
  data
• System with silicon photomultiplier has a good
  sensitivity and can be used to measure
  fluorescence light intensity for fluorescein
  concentration from 1ug/ml to about 60pg/ml and
  resorufin at the level of 1ng/ml up to 1ug/ml.

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Future work

• Stabilization system compensating temperature
  influence on SiPM gain by varying supply voltage
• Measurements of fluorescent dyes in
  microbioreactors.

scanning probe
micro-bioreactor
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Thank you for your attention