Micropipet

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Micropipet
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How to use a micropipet

• In science, there are many different ways to
  measure volume.  One very important tool to a
  molecular biologist is a micropipet.  A
  micropipet measures in micro liters ( millionths
  of liters).  Some of the pipet measure very small
  volumes and some measure larger volumes.  Each of
  these pipettes must have a disposable pipet tip
  on them.

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The micropipet
Video on how to Adjust Dial
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• How to place a tip on the pipet
• Take the pipet and place into a tip in the tip
  box.

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Press Down firmly but not too hard.  Tap a few
times to seat the tip
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Yeah! Good Job.
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How to Use the Pipet to Load a Gel

• Press the plunger down to the first soft stop. 
  You will feel it stop naturally

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Lower the tip into the loading dye and slowly let
the plunger come back up and fill the pipet.
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Pick up the sample that you are adding loading
dye to and lower the pipet into it.
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Press down to the first soft stop to deliver the
loading dye.  If it all doesn't come out you can
push down to the hard stop to give it one last
chance to come out.
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Important!!!!!!

• Important! Don't lift up on the plunger until
  after you have pulled the tip out of the sample
  tube.

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How to Eject a Tip Once you have Used itTip is
ejected by placing finger on Tip Ejector
ButtonPress Down on Ejector Button Holding the
pipet over a waste cup
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• Video on how to eject a tip

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