OVERLORD

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Introduction

• OVERLORD
• Labcyte Echo 550 Nanolitre Dispenser
• Extending functionality using OVERLORD
• Integrating the Echo into an automated system

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OVERLORD

• PAA offer two software products to automate Echo
  550
• OVERLORD Workstation
• OVERLORD Scheduler

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OVERLORD Workstation

• Icon based drag and drop set-up
• Flow chart interface
• Very flexible
• Dynamic Scheduling
• Decisions can be made in real-time

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OVERLORD Scheduler

• Icon based drag and drop set-up
• Gantt chart interface
• Optimise/Interleave assays in single run
• Each assay run with exact timings
• Very easy to create an assay
• Add new assays when system is running

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Common Features

• Support for over 200 instruments
• Uses original instrument software when possible
• Automatic data handling
• Can integrate with most databases (e.g. SQL
  Server, Oracle, Microsoft Access etc)
• Optional 21 CFR Part 11 compliance
• Full system tracking
• Error notification
• Error recovery

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Labcyte Echo 550

• Transfers volumes down to 2.5nl
• Uses touchless technology
• Based on focused acoustic energy
• Can measure the water content of DMSO and the
  fluid depth in each well

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Labcyte Echo 550 Functionality

• Plate to Plate Transfers (Quadrant or
  Interleaved)
• Plate Surveys
• Use 384 or 1536 well plates
• Protocols defined in the Client software
• Data reported in XML

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Functionality with OVERLORD

• Plate to Plate Transfer - Interleaved or Quadrant
• Plate Survey Includes reporting sample volume
• Cherry Picking software module available
• IC50 software module available
• Use 384/1536 plates OR 96 well destination plates
• Protocols defined in OVERLORD
• Data reported in any format (CSV, Excel, XML,
  Write to SQL Server, Oracle, etc)

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Integrating into a Workcell

• Labcyte Echo 550 can be integrated with nearly
  any device
• Robotic arms such as the automate.it KiNEDx arm
  can be used
• All fully controlled from OVERLORD

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Summary

• OVERLORD Workstation and Scheduler
• Functionally of Labcyte Echo 550
• How OVERLORD can increase the functionality of
  the Labcyte Echo 550
• How OVERLORD can integrate the Labcyte Echo 550
  into an automated system with other equipment

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Any other questions / points? All trademarks
acknowledged