Enterotube

1
Enterotube
See pages 56 57 in your Lab Atlas.
2
Enterotube
Gas production Wax separates from agar OR
air bubbles in agar
3
Enterotube
Indole test
4
Enterotube
Indole test 1. Melt film on bottom of
compartment. 2. Add 2 drops Indole reagent.
(on back table)3. Wait 10 sec. red positive
5
Enterotube
Phenylalanine test
6
Enterotube
VP test OMIT
7
Enterotube
Understanding the Enterotube interpretation
table
8
Enterotube
The numbers on the Interpretation Chart represent
the of strains of that organism that show a
positive result.
90 positive 10 but 90 test
UNRELIABLE for this organism 10 negative
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Redundancy of tests

• e.g. The ability to ferment lactose is tested
  in
• Brilliant green agar
• EMB agar
• Enterotube

If you are suspicious about the results of
lactose fermentation test in the EMB plate, check
lactose fermentation in the Brilliant green agar
plate and in the Enterotube.
10
Levine Eosin-Methylene Blue (EMB) Agar
11
Brilliant Green Agar
E. coli
Salmonella