DNA???(DNA binding domain,DB);

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???????????????
2005.9
???? ?????
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• ???????,????????????????
• ????????????????????????????????????????????????
  
• ????????????,????????????????????????????????????
  ????,????????????,???????????????????

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?????????????????????
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?????????????

• ????????????????????
• DNA???(DNA binding domain,DB)
• ????(??)?(transcription regulation
  domain)???????(transcription- activating
  domain,AD)????(repression domain)?????????(nuclear
  localization signals,NLS)
• ?????????????(dimerization domain)?????????????,?
  ???????????

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• ???DB?????????,????????
• ???????DB?AD?????????????????????
• ????????????????DNA???????????

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DNA???
DNA????60-100????????,????????,??????????
???(zinc-containing modules)?????(homeodomain,HD)?
??-?????(basic-leucine zipper,bZIP)???-??-?-??(bas
ic-helix/loop/helix,bH-L-H)?MYB????????
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• ? ????
• ???3??????DNA???,??????????????????????????
  ?????,???????????a??,??DNA?????????
• ??????1)??2)???????????????3)????GAL4,?
  ??2?????6????????

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? ??????? ???60????,?????-??-??(helix
-turn-helix)DNA????????????????????????????(homeob
ox)???????HD????????(homeobox gene)???????????????
???????????????????HD???????????????????????,????K
N1?HD-Zip?GLABRA2?PHD-Finger?BELL1?????????-??????
?(homeodomain-leucine zipper protein,HD-Zip)??????
????????????????????????????
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(No Transcript)
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? ??-????? ??????????????????6????????
?????,??????????????a?????????????????????????????
????????,????????????????????,??????DNA???????????
???????????????DNA???????????
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? ??-??-?-?? ? MYB???
MYB???????5153?????????,???????????????????,?????
18??????????????(W)??,????????????????MYB????????-
??-??-??(helix-helix-turn-helix)??,???????????????
????????
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• ?????DNA???????,???????????????DNA????,?????????
  ??,????????DNA????????????????????????????
• ????????????????,??bZIP?????1?????????????1??2?
  ??????????????????????????
• DNA?????????????????????????????,???????????????
  ?????????????DNA??????????????

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• ????DNA????????????????????????,????????????????
  ??????????

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???????????????DNA???,????????????????Myb
???????Myb????????,???????????????Myb????3????????
??,?????????5153????,???3???????1819??????????,?
??????????????????DNA???????,????????????????Myb??
?????????????3?a??,???3??????????
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?????,???????Myb???????,????????????????My
b?????????????,?????Myb????????????????
?????,??Myb??????????????????????????????????????G
A???????????????????????Myb???myb????
??????????,?????????????????????????????
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• ?????????????????DNA???,???????????????????
• VP1???????(?????Em??)?????,???????????DNA???-BR2(
  B2)???????????BR3(B3),????Sph??(CATGCATG),???????E
  m????????????
• ?????,VP1???BR2??BR3???Em???,??????BR2?????14-3-
  3???????,???????????(?)???????????????14-3-3????D
  NA???,????VP1????????????,?EmBP1(Em Binding
  Protein 1)?Em??????????????

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??????????????-1(ethylene-responsive
element-binding protien-1)????DNA?????????????1(CO
P1)??G??ß?????bZIP??PEND???????????????,??????????
??????
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????? ??????,????????????????????
?,???????????????DNA??,???????????????????????????
?,?????????????????????,????????????(repression
domain)?
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????????????,??????????,??????DNA??????30
100??????,???????????????????????????????????????
???????,?????????????????????????????
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? ?????a???? ????????????????????????,?
?AP1???Jun?????a????,??????????????????
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? ????????? SP1?????GC??????,??4??????
????,??????????????,???????? ????????Oct1/2?Jun?AP
2?SRF?????????????????????
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? ???????? CTF-NFl???C??????(?2030),
????a???????Oct2?Jun?AP2?SRF??????????????????????
? ????????a???????????????
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???????????????????? ????????,??
Myb?????C1????11?????????1?256????????????,253????
???????????,???????????????????????,??????????????
??????????????????VP16????????TF?B????????????????
???????????
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??????? ????????????/????????,???DNA????
????????????????????????
????????ß-????????????????????????????
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????????????????????????,????????DNA???????
????????????bZIP??????????????????????????????????
?,bH-L-H????-?-????????,?MADS????????????2?a-???2?
ß-?????? ???????????????????????,???bZIP??
????????????4??5?7????,??????ATB2???9??
????????????????????????
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??????? ???????????????????,?????????????
?????????????NLS,?????????????????????????NLS?????
????????,???????NLS????????????????????
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????????????????? ???????????????????????
????????????????????????Cys(C)?
His(H)??????????????5?C2H2?C3H?C2C2(GATA?),C3H4(?
?(ring finger))?C2H5(LIM?)
????????????????????????????,?????????????????KN1?
HD-Zip?GLABRA2?PHD-Finger?BELL1???
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??????????? ????????????????????????????
?????,?????????????????????????????,??????????????
????????????????????????
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????????????????????????,?????????????????
?????????????????????????????????????????????????
?????????????bZIP??OsZIP-2a?2b,????????bZIP????EmB
P1?????,??????????????????????????????(repression
domain)????
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????-1(Yin Yang-1,YY1)?????????,??????????
???????????????,??YY1?????????,???????????????????
??? ??YY1???????? CCAT?ACAT?TCAT,?????????
?????,????????????????YY1?????????????,???????????
YY1?????????????????????????,????????????
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???????????? ????????????
???????????????????,??????????????????????????????
???????????????????????????????????,??????????????
???,?BnGBF1a?1b?2a 3?GBFs?????????????????????(1agt
2agt1b),????????????????mRNA?????
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???????????????????,???????65
kD?DNA????Rel A?????50 kD?p50?????????NF-?B
???????????????????mLIP15(maize
low-temperature-induced protein 15)?1?bZIP
??,??????????????ABA????????????,?????????????????
?????, HD-Zip?????????????????,???????ABA????????,
?????????????????????????
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????????????????????? ???????????????????
,????????????????????????????????????,??????mRNA??
??,????????????,??????????????????????????????????
???????Myc????????Atmyc1???????????????Sph?(CATGCA
TG)?????????????????????,???????????????????????,?
?????????????????????????
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?????????????? ????????????????????mRNA??
???,???????????????????????????????P??????mRNA??3
????????????,???????1802 ????????43.7
kD???,?N????Myb???????DNA????40
???????????945???,????17.3kD???,????Myb??????????
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???????????????? ???????????????,????????
?????????????????????????NLS???,NLS???????????????
????????,?bZIP???HY5(hypocotyl 5)?????????,???????
?????????????????,?????????????????(cytoplasmic
retention factors),?????????NLS??(intra- and
inter-molecular NLS masking),??????NLS????????????
???
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???????????DNA??????? ?????????DNA???????
???????????/?????????????????,????????????????????
DNA??????????????????,???????????????????O2???????
???????????????DNA???
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????,?????????????????????DNA???,????????
?????????????,????????????????????????????
???????????????,??bZIP????HBP-1a?DNA?????????????
????Ca2?,????bZIP??Opaque 2(O2)??????????(circadi
an-clock-related)??????????,??ZmMYBP???????DNA????
???ZmMYBP????????,??MYB?????????????????????Cys???
????????????
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?????(IN)???/???????????????????????DNA???????????
?????? NF-?B???P65?N??????300?????????Rel?
????(RHD),RHD????DNA????NLS?????????
I?Ba?NF-?B ???????,?????????I?Ba?NF-?B
?????????RHD??NLS,NF-?B????????????
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??????????????????????(combination control)
??????????????????????,???????????????????,????
???????????????DNA?????????, ?????????
RNA????????????????????(transcription
machinery),????????????????????????????,??????????
???,?????????????
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?????????????????????
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?????DNA???(???????????????)??????????????
????????????????? ?????????????????,???????????,??
??????????????????????
?????????????DNA??????,??DNA??????????????????????
??????DNA,??????????????-????????DNA???????????,??
DNA???????????DNA???,????????????????????,????????
?
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Thank you for your patience!