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tawan_at_mjuacth
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???? (Introduction)
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???? (Introduction)
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???? (Introduction)
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Parallel Fermentation Facility
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BioFlo 6000, 130 Liter capacity fermentor. Shown
with open skid equipped with optional automatic
sterilization feature.
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1. Microbial cell and Biomass
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1900 ??????? ??????????????????????????????????
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??????? ?.?. 1960 ??????????????????????????????
?????? (SCP) ?????????????????
Microbial cell and Biomass ????????????????
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1. Microbial cell and Biomass
There may be biological waste treatment in larger
vessels, but the world's largest fermenter is
shown in these photos taken from Chemical and
Engineering News. The fermenter is 200' high and
25 ft diam. The first photo (Chem. Eng. News,
10-Apr-78) shows the fermenter being transported
on vehicles with tank treads.
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1. Microbial cell and Biomass
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Air-lift ???? 1,500 ??.?. ????????????????????????
???????? 54,000-70,000 ???/?? ????????????????????
??
Methylophilus methylotrophus I.C.I. Methane
from natural gas or methanol
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2. Microbial Enzyme
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Enzyme Amylase Protease Pectinase Cellulase
Isomerase Invertase and Oxidase
Fungi and Bacteria
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2. Microbial Enzyme
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3. Microbial metabolite
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??? - ???????????????? (Primary Metabolite) -
?????????????????? (Secondary Metabolite)
???????????????? (Primary Metabolites)
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3. Microbial metabolite
?????????????????? (Secondary Metabolites)
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3. Microbial metabolite
cell constituents Nucleic
acids Proteins intermediates
Polysaccharides Nucleotides Lipids Amino
acids Sterols Sugars
secondary metabolites Acetyl
CoA Penicillins, Cephalosporins Terpenes p
eptide antibiotics, alkaloids Aminoglycosid
e antibiotics Tetracycline
antibiotics Gibberellins
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4. Recombinant products
?????????????????? Recombinant DNA
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?????????????? ???????????????????????????????
?????? Interferon, Insulin , Human serum
albuminGrowth factor, Calf chymosin, bovine
somatostatin ???????
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5. Biotransformation
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???????????????? Prostaglandins ?????????????
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1. The formulation of media to be used in
culturing the process organism during the
development of the inoculum and in the
production fermenter. 2. The sterilization of
the medium, fermenters and ancillary
equipment. 3. The production of an active, pure
culture in sufficient quantity to inoculate the
production vessel.
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4. The growth of the organism in the production
fermenter under optimum conditions for product
formation. 5. The extraction of the product and
its purification. 6. The disposal of effluents
produced by the process.
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???????????????????
Q1 What are the differences between
Biotechnology and Fermetation Technology?. Q2
What are the steps of Fermentation Technology?.
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See you again next Thursday on the topics of
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