General user guidelines

1
General user guidelines

• New users
• Notify the lab (lab_at_mmb.bme.wisc.edu)
• Go over lab protocol slides
• If in doubt, ask
• Sign up for equipment time on WiscCal (see Jay),
  notify others if you will be unable to use your
  time
• Replace items when taking the last one
• Make sure that supplies are reordered before
  running out
• Refill pipette boxes, solvent bottles

2
General user guidelines

• Label all chemicals
• Remove materials and clean common areas
  (microscope, hoods, etc.) after finishing work
• Leave a note/check with other users prior to
  borrowing equipment (e.g. radiometer)
• Promptly return equipment when done
• The room should like it was when you entered

3
3006 - Hoods

• organic SOLVENT and ACID/BASE in separate hoods
• Solvent acetone, methanol, IPA
• Keep carboy closed at all times
• Keep sash windows shut when not using

4
3006 Types of Trash

• Monomer Contaminated Glass
• Rinse with solvent into carboy (keep closed at
  all times)
• Once dry, place in broken glass container
• Chemical Contaminated Labware (kimwipes, pipette
  tips)
• Put in Oily Waste yellow container
• When full,close bag and label Contaminated
  Labware, monomer, plastic pipettes, paper
  products

5
3006 Acticure

• If left on, note when you will be done using for
  the day
• UV must be on for at least 20 minutes, can be
  left on during the morning
• Do not adjust settings without permission while
  others are logged in
• Dispose of dirty pipettes, paper towels, etc.
• Leave fan on for 15 minutes after turning off UV

6
3041

• Spinner
• Take out chuck before cleaning
• Replace aluminum foil after you finished
• PDMS Scale
• Clean up around and top of the scale after
  measuring and mixing
• Dispose of unused PDMS or let others finish
  excess
• Use separate dishes for first/second development
  steps
• Save fresh SU-8 developer
• Hood
• Keep sash windows closed
• Keep solvent carboy closed

7
2046 Cell Trash

• Cell contaminated devices or glass (no virus, or
  recombinant stuff)
• Spray with some 1-10 bleach solution
• Put in broken glass disposal
• Large amounts of cell waste should be dealt with
  elsewhere and should be autoclaved!

8
Other Instruments in labs
Spectrophotometer
Analytical Balance measure up to 0.1 mg max
50 gm Static brush clean after use
Drill press
Syringe Pumps
Goniometer
UV sources switch off when not in use leave
off for 15min b/f restart Log usage
pH meter
Please keep them clean and in working condition
9
Sharps disposal in labs

• Sharps - JUST needles with syringe bodies
• Do not recap needles
• Expose any prepolymer mixture before putting in
  sharps container
• Do not overfill the sharps container
• Call Chemical safety to come pick up the boxes
  when full
• Make sure to get a replacement sharps container -
  Fisher sells them

10
Water in labs

• RGW Reagent Grade Water
• General purpose
• DI Water Nanopure, 18 Mohm
• Tap water
• Make sure that you refill with the right water