???????L??????

1

• ???????L??????
• Streptolysin O?
• Streptokinase?????? -
• Pathogenicity of Streptococcus pyogenes L-form
• -Productivity of Streptolysin O and
  Streptokinase-

2
1
????????????????L????????????????L????????????????
???????????L???????????????????????????L??????????
??????????????????????????????????????????????L???
???????????????????????????????????????????L??????
?????????????????L??124L??????????O ??????????
??????????????79?????????????????????????????124L?
???124????????????????????????????????????????????
3
2
???????????? ??L ??
L ??124 L ???? 82????????13??L????? ?????????????
?L???(????????1972?)
4
3
???????? L ??124 L ?
1µm
0.05 µm?µm????????
5
4
????????????L???124 L??????124L????124?(?????????
????)?K6169??K5866?(??????????????)??????????????
??(????)?????????????????1.5ml????37???????????L??
?????(0.5 yeast extract, 4 NaCl, 10
FBS????brain heart infusion broth
)?????????????????????????????????????????????????
???????SLO??(ABS? HAB003)??????????????????SK
??(ACR? BP988)???????????????????
6
5
?? 1 ???????L??124L????????????????(3.5NaCl)????
??
?????124L??????????????
7
6
?? 2 ???????L??124L????????????????(3.5NaCl)????
??
??????124L??????????????
8
?? 3 ???????L??124L??????????O??????????????????
?? Anti-streptolysin O mouse monoclonal antibody
(1250??)
7

124L????SLO??????????????????????
9
8
?? 4 ??????? L ??124 L ?????????????????????????
??? Anti-streptokinase rabbit polyclonal antibody
(112000??)
124L????SK??????????????????
10
9
? ? ? ? ?
1.???????????(124L???????)?? ???????? O
????????????????????L?????124 L
?????????????????? 2.???????? L
???????????????????????????????????????? 3.??????
??????????????L????????????SLO????SK??????????????
????????????????????????
Wild types of S. pyogenes produced streptolysin
and streptokinase, but L-form of 124L strain did
not produce both enzymes.
There is a possibility that L-forms of S.
pyogenes lacking in production of both enzymes
emerge in vivo.
Changes wild types of S. pyogenes into L-forms
might explain ineffectiveness of Penicillins and
negative findings for anti-SLO and anti-SK
antibodies in patients with Kawasaki disease.
11
???79???????????????????????SLO?????
??? ??????? L ??124 L ?? ????????O
????? ?????????????(?????????)
L??????????????????? ?????L??????????????????
??????
L??????? (0.5 yeast extract, 4 NaCl, 10
FBS????brain heart infusion broth )
12
?? (????)
? ? ?
L ? ?
Control
K5866
K6169
124 L
124 L ??????( )????????
13
?? ???????????
124 L???????????????????????
14
?? ?? L ?????? SLO ????????? - L
????????????????????-
???
L ? ?
???? L ??
????????
??????
???????
?????? ???????????
?????????
???
L ????????????????????????????????????????????????
??????????????????????????????????????????????????
?
15
??
???
?????????????????? 1.??????????????L????????? 2.
???????????ASLO??ASK??????? 3.?????????????
16
????L???????? 1.??????????????? L?????????
???????????????????????? ????????????????(?????)?
?????L?????????????????? ????????????? 2.?????
??????? 3. L ?????????????????????
17
?????????????????????
??? Cater??1975
??? Ueno??1983
??? Ueno??1983
??? ????1985
18
??? (Ueno? 1983)
L??124L? (????1979)
??????????????L?????????????