DNA Sequencing

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DNA Sequencing

• A population of DNA fragments is generated.
• One end is common to all fragments (the 5 end of
  the sequencing primer).
• The other end terminates at all possible
  positions (the 3- terminus).

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2',3'-Dideoxyribonucleoside Triphosphates
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Sanger Sequencing
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Automated DNA Sequencing

• Fluorescent dyes are used for detection of DNA
  chains instead of radioactive isotopes.
• Products of all four chain terminator reactions
  are separated through a single gel or capillary
  tube.
• Photocells detect fluorescence of the dyes as
  they pass through the gel or capillary tube.
• Output of the photocell is directly transferred
  to a computer for analysis.

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Next Gen
http//www.youtube.com/watch_popup?vbFNjxKHP8Jcv
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tch_popup?vg0vGrNjpyA8vqmedium
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Linker scanning the old fashioned way
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Two Hybrid
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One-Hybrid
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