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Title: The Keys to Laboratory Safety: Semester Review


1
The Keys to Laboratory Safety Semester Review
  • Bill Diesslin
  • March 3, 2009

2
Chemical Safety in the LabRisk Management
Name two federal agencies that have regulations
that deal with chemical use in the laboratory.
3
Chemical Risk Management
What is the name of the document that is the
primary resource for information on how to use
chemicals in laboratories at ISU?
4
ISU Laboratory Safety Manual
  • Introduction
  • Process Planning
  • Emergency Planning
  • Equipment
  • Training
  • General Lab Safety Practices
  • Safety Practices for Specific Hazards
  • Management of Unwanted Lab Materials (Waste)
  • Exposure Assessment and Medical Care
  • Appendix I Forms
  • Appendix II Lab Related Manuals

5
ISU Laboratory Safety Manual
  • YOUR RESPONSIBILITIES
  • Familiarize yourself with the document
  • Be aware of lab hazards
  • Follow Standard Operating Procedures
  • Review processes with major professor
  • Use equipment properly (i.e., PPE, fume hoods,
    etc.)
  • Label, store dispose of chemicals properly
  • Manage chemical inventories
  • Be a good example to others

6
Personal Protective Equipment
Chemical-resistant gloves are commonly used in
the laboratory. Name 3 things to be aware of
when selecting and using chemical-resistant
gloves.
7
Personal Protective Equipment
  • PPE is last line of defense
  • Identify the hazards
  • Selecting the right PPE
  • Types available
  • Minimum required in a lab
  • Special cases
  • Your responsibilities when wearing PPE
  • Regulated by OSHA
  • Part of SOPs

8
Chemical Hazard Information
Name two sources of chemical safety information.
9
Chemical Hazard Information
  • Constituents of MSDS
  • Health physical hazards
  • Controls
  • Label requirements secondary containers
  • Relative to use
  • Allows hazard assessment

10
Chemical Hood Usage
Name three things to keep in mind when using a
chemical hood.
11
Chemical Hood Usage
  • Purpose
  • Operational Characteristics
  • Proper Hood Usage
  • Common Usage Problems
  • Servicing

12
Laboratory Inspections
Names three things that would be deficiencies
listed on a laboratory inspection form.
13
Laboratory Inspections
  • POSITIVE IMPRESSIONS
  • Every occupant wearing PPE
  • Door has current emergency contact information
    and NFPA sign
  • No food or drink containers in laboratory
  • Room is clean and uncluttered
  • Eyewash station is clean and labeled as being
    tested
  • Chemical containers are from the 21st century
  • All glassware is clean and put away when not in
    use
  • All chemical containers has labels with names
    spelled out
  • Centralized location for all documentation
    (Laboratory Safety Manual, MSDSs, training
    records, emergency contact info)

14
Hazardous Waste Disposal
The new term used to refer to waste is _________
________.
15
Hazardous Waste Handling at ISU
16
Chemical Storage
Peroxide-forming chemicals must be tested how
many months after opening of the container?
17
Chemical Storage
  • Why is proper storage critical?
  • Labeling
  • General Storage Considerations
  • Storage in Refrigerators
  • Storage in Fume hoods
  • Flammable Storage

18
Hazardous Materials Shipping
Name the U.S. authority for all shipments by
land, sea and air.
19
Hazardous Materials Shipping
  • What materials are regulated for shipping?
  • What can happen if you dont comply with the
    regulations?
  • What are the steps involved in shipping?

20
Biosafety
What is the name for an organism that causes
diseases in humans, plants or animals?
21
Biosafety
  • Recombinant DNA
  • Transgenic Animals or Plants
  • Bacteria, Viruses, Fungi, Prions
  • Biological Toxins (e.g.Tetanus Toxin)
  • Human Blood and Certain Body Fluids
  • Institutional Biosafety Committee approval

22
Emergency ActionsChemical Spill Procedures
Name two things you would find in an emergency
action plan.
23
Emergency ActionsChemical Spill Procedures
  • Types of emergencies
  • Procedures
  • Alarm systems
  • Chemical spill issues
  • Mercury
  • Small versus large?
  • Part of employee training

24
Electrical Safety
Name two types of electrical shock injuries and
two factors impacting electrical shock.
25
Electrical Safety
  • Improper use of electrical equipment.
  • Damaged equipment cords, plugs.
  • UL-approved
  • GFCI
  • Vapors

26
Fire Safety in the Lab
Name the three components of the fire triangle.
27
Fire Safety in the Lab
  • Fire Chemistry and Principles
  • Controlling Lab Fire Hazards
  • Fire Evacuation Procedures
  • Fire Extinguishers
  • PASS

28
Common State Fire Marshal Fire Safety Citations -
ISU
  • Hallway doors opened
  • Storage lt 18 below sprinkler heads
  • lt 3 clearance by electric panels
  • Improper use of extension cords door wedges
  • Lack of orderly and clean laboratories

29
Pressure Safety
As volume increases, pressure __________ as
temperature increases, pressure __________.
30
Pressure Safety
  • MAWP
  • Safety Factor
  • Hazards of cryogens?
  • Cylinders primary/secondary hazards

31
Radiation Safety
Radiation that has enough energy to cause the
release of an orbital electron is called
__________.
32
Radiation Safety
  • Radiation Types
  • Ionizing
  • Non-Ionizing
  • Controls
  • ALARA

33
Laser Safety
Name 3 ways that you can prevent exposure to a
laser beam.
34
Laser Safety
  • LA-SER
  • Which classes of lasers are hazardous?
  • Which non-beam hazard is the most significant?
  • How do you protect yourself when using a laser?

35
Thats It!
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