DNA Fingerprinting Agarose Gel Electrophoresis Student Instructions

1
DNA FingerprintingAgarose Gel
ElectrophoresisStudent Instructions
2
Agarose Gel Electrophoresis Step 1

• Use the pipettor to add 4 µl of migration dye
  from tube "D."
• Make sure to change tips after each use.

DNA Fingerprinting Student Instructions
3
Agarose Gel Electrophoresis Step 2

• Using a pipettor set at 20 µl, transfer the
  sample into the appropriate lanes of the gel.
• Make sure to change tips after each use.

DNA Fingerprinting Student Instructions
4
Agarose Gel Electrophoresis Step 3

• After the samples are loaded, slowly fill the gel
  box with the 1X TBE buffer. Make sure the gel is
  completely covered.
• If there is not enough buffer, add distilled
  water to finish covering the gel.

DNA Fingerprinting Student Instructions
5
Agarose Gel Electrophoresis Step 4

• Turn the switch on the power supply to "Off"
  before connecting the electrophoresis chamber.
• Place the lid tightly on the chamber and plug the
  electrical leads into the recessed output jacks
  of the power supply.
• Plug the red () lead into the red jack, and the
  black (-) lead into the black jack.

DNA Fingerprinting Student Instructions
6
Agarose Gel Electrophoresis Step 5

• Select the desired voltage on the power supply. A
  voltage of 150 will permit the electrophoresis
  run to be completed in about an hour.

DNA Fingerprinting Student Instructions
7
Agarose Gel Electrophoresis Step 6

• Turn the power supply switch "ON." The blue
  migration dye should move toward the positive
  electrode (red). If it is migrating toward the
  negative electrode (black), turn off the power
  supply, remove the lid, turn the gel tray 180o,
  replace the lid, and turn the power supply "ON."

DNA Fingerprinting Student Instructions