Fish DNA Barcoding Game! A Jeopardy-Style Quiz Game

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Fish DNA Barcoding Game!A Jeopardy-Style Quiz
Game
DNA Extraction PCR Gel Electrophoresis Not Like the Other Miscellaneous
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Inc.
2
Answer

• An organelle other than the
• nucleus that contains DNA.

3
Question

• What is a mitochondrion?

4
Answer

• All of the chromosomal
• DNA found in a cell.

5
Question

• What is genomic DNA?

6
Answer

• The process of rupturing
• a cell to release its contents.

7
Question

• What is lysis?

8
Answer

• A suspension that traps
• impurities from fish tissue
• and prevents contamination
• of DNA.

9
Question

• What is matrix?

10
Answer

• The liquid portion above the pellet
• formed after centrifugation.

11
Question

• What is the supernatant?

12
Answer

• Of the four PCR reactions
• in your table setup today,
• this is the sample that
• doesnt have any DNA in it.

13
Question

• What is the negative control?

14
DAILY
DOUBLE

• How much do you wish to wager?

15
Daily DoubleAnswer

• An enzyme used in PCR
• that is able to function at
• very high temperatures.

16
Daily DoubleQuestion

• What is Taq polymerase?

17
Answer

• These target the portion
• of DNA to be amplified.

18
Question

• What are primers?

19
Answer

• A step in PCR in which the
• temperature is lowered to
• 5065ºC, allowing the
• primers to bind to the DNA.

20
Question

• What is annealing?

21
Answer

• The complementary strand
• of the ACTGGTTA template
• strand that is being copied
• during extension.

22
Question

• What is TGACCAAT?

23
Answer

• The name of the gene
• that is being amplified
• by PCR in the
• Fish Barcoding Kit.

24
Question

• What is COI?

25
Answer

• This acts as a sieve to
• separate DNA based on
• its size when an electrical
• field is applied.

26
Question

• What is an agarose gel?

27
Answer

• The size of the amplified
• portion of the COI gene.

28
Question

• What is 650 bp?

29
Answer

• It weighs down the
• DNA sample so that it stays
• in the well after loading.

30
Question

• What is loading dye?

31
Answer

• Its used to determine the size
• of the DNA (in base pairs)
• in an agarose gel.

32
Question

• What is a DNA ladder or
• molecular weight ruler?

33
Answer

• Where negatively charged
• DNA migrates to during
• gel electrophoresis.

34
Question

• What is the positive or
• anode side of a gel
• electrophoresis chamber?

35
Answer

• P1000
• P200
• P50
• P20

36
Question

• Which of these is
• not an actual pipet?

37
Answer

• DNA extraction
• RNA extraction
• PCR
• Gel electrophoresis

38
Question

• What is RNA extraction?
• (not part of todays experiment)

39
Answer

• Cod
• Trout
• Catfish
• Mussel

40
Question

• What is mussel?
• (not likely to work with fish barcoding kit)

41
Answer

• Denaturation
• Annealing
• Extension
• Sequencing

42
Question

• What is sequencing?
• (not a step in PCR)

43
Answer

• Thermal cycler
• Cathode
• Anode
• Electrical current

44
Question

• What is a thermal cycler?
• (not used in gel electrophoresis)

45
Answer

• The identification of an
• organism at the species level
• using a several hundred
• base pair sequence of DNA.

46
Question

• What is barcoding?

47
Answer

• The order of bases that
• make up a DNA strand.

48
Question

• What is a sequence?

49
Answer

• The database used to
• identify a mystery fish
• at the species level.

50
Question

• What is BOLD?

51
Answer

• A group of organisms
• capable of interbreeding and
• producing fertile offspring.

52
Question

• What is a species?

53
Answer

• A place where specimens
• are stored to support future
• scientific investigations.

54
Question

• What is a biorepository?