Body Fluid ??

1
Body Fluid ??

• ??????

2
??

• CSF
• Synovial Fluid (???)
• Pericardial Effusion (????)
• Peritoneal Effusion (????)
• Pleural Effusion (??)
• Ascites (??)
• other

3
CSF

• Apperance
• Pandy test
• Specific Gravity (SG)
• Cell count
• Differential count (DC)
• Gram stain???

4
Apperance

• Clarity (???)clear?turbid
• Color (??)colorless?yellow?red?
• Clot ???

5
Pandy test

• ??CSF???Protein
• 1ml?10 Phenol1?CSF
• ()?????
• (--)????

6
Specific Gravity (SG)

• ????dis water??????,?? ?,??1?CSF,??
• ???1.0051.007

7
Cell count\DC

• Cell count
• ??CSF?WBC???
• ????CSF,???????,??9???WBC???
• DC
• ??WBC?PMN?Mononuclear???
• ??Cytospin Auto Smear,?1500rpm-5min,??Liu,s
  stain,??

8
Normal CSF DC count
Cell type ??? ??
Lymphocytes 70 20
Monocytes 30 70
Neutrophils 2 4
9
Synovial Fluid (???)

• ????
• Appearance
• Cell count
• Differential count (DC)
• Viscosity
• Fibrin-clot formation
• ??

10
Cell count of Synovial Fluid

• Wash
• ???Specimen,??
• ??????,???Normal saline,??,??
• ??(????)23?,????
• Diluent
• ?HCl?diluent?5x?10x?20x
• ????Counting chamber,??4??
• 4??cell X 2.5? X diluent?? /cmm

11
??????WBC??
WBC?? ???
????? 025
??? 078
??? 071
???? 026
12
Pericardial?Peritoneal Effusion? Pleural
Effusion?Ascites

• ????
• Appearance
• Rivalta,s test
• Specific Gravity (SG)
• Cell count
• Differential count (DC)
• ??

13
Rivalta,s test???

• ?100ml water???2?acetic acid,????2?sample
• ??????50ml (2/3)??,?Positive
• Transudate--Negative
• Exudate--Positive

14
???(Trnasudate) ??? (Excudate)
Color ?????? ?????????
SG lt1.015 gt1.018
????? lt3.0 gt3.0g/dl
???(??/??) lt50 gt50????
LDH??(??/??) lt60 gt??60??
Fibrinogen 0.34.0 46???
Clot?? ???? ?????
RBC ??????? ?????????
WBC ??????? ?????????
Glucose ?Plasma???? ????Plasma??(lt60mg/dl)
D.C ??????????
Rivalta test ?? ??
??? (-) ??lt50Serum,??????
15
Thank you