BODY FLUID ANALYSIS FOR CELLULAR COMPOSITION

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BODY FLUID ANALYSIS FOR CELLULAR COMPOSITION

• Afsaneh Rajabiani, APCP
• Tehran Medical University
• Shariati Hospital

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Morphology Assessment of Body Fluid

• Wedge smears (push smears) should not be used
  with fluids because of their inferior ability in
  preserving intact cells.
• The cytocentrifuge preparation is recommended for
  air-dried body fluid slides, because
• Concentrate the cells
• Minimizes cell distortion
• Produces a monolayer of cells
• Romanowsky-type stained slides show excellent
  morphologic detail
• Cell typically are randomly dispersed in a small
  circular area
• When malignancy is suspected, the whole cellular
  area should be evaluated

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Morpholoy Assessment of Body Fluid

• Cells are concentrated approximately 20-fold by
  cytocentrifugation
• Even hypocellular samples with a chamber cell
  count of zero can have a yield of approximately
  35 cells per slide.
• The quantitative yield, however, varies from 30
  to 75, and smaller cells, such as lymphocytes,
  may be underrepresentated
• The speed and time of centrifugation, the amount
  of sample in the chamber, and the filter paper
  absorbance are factors that can influence both
  the cell yield and morophology

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Morphology Assessment of Body Fluid

• Fresh, unfixed specimens should be used,
  particularly in body fluid samples with low
  protein contents (such as CSF)
• Prolonged delay in preparing slides (more than 8
  hours), the report should include a statement
  that the differential count may be inaccurate,
  due to cellular degeneration
• Body fluids may contain fibrin and other proteins
  that can clog the filter card
• Washing the cells by centrifuging the sample and
  resuspending in saline, improve the cell yeild
  and morphology

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