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Drosophila Genetics

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Drosophila Genetics Inheritance of Body Color B. Three Point Testcross Inheritance of Body Color: Phenotypes of Parents Inheritance of Body Color: Crosses A x C B x C ... – PowerPoint PPT presentation

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Title: Drosophila Genetics


1
Drosophila Genetics
  • Inheritance of Body Color
  • B. Three Point Testcross

2
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3
Fruit Fly Egg and Larva
4
Fruit Fly Pupa and Adult
5
Inheritance of Body Color Phenotypes of Parents
Letter Gender Body Color
A Female Beige body
B Female Beige body
C Male Beige body
D Female Yellow body
E Male Yellow body
6
Inheritance of Body Color Crosses
  • A x C B x C D x C
  • A x E B x E D x E
  • Score each fly for gender and body color

Yellow
Beige
7
Distinguishing Male and Female Fruit Flies
  • Male body size is smaller than female
  • Male abdomen is tapered and darker than
    female abdomen
  • Males have sex combs at first joint on front legs

You MUST be able to see sex combs on any fly you
identify as male!
8
Three Point Testcross
9
Three Point Testcross
10
Three Point Testcross Possible Phenotypes
Body Color Wing Length Eye Color
Beige Long Red
Black Vestigial Brown
Beige Vestigial Brown
Black Long Red
Beige Long Brown
Black Vestigial Red
Beige Vestigial Red
Black Long Brown
11
Three Point Testcross F2 Offspring
  • Score each fly for body color, wing shape and
    eye color.

12
Wild Type Versus Mutants
  • Body Color
  • Wild type Beige
  • Mutant Black

13
Wild Type Versus Mutants
  • Wing Shape

Wild Type Long
Vestigial
14
Wild Type versus Mutant
  • Eye Color
  • Wild Type brick red
  • Mutant Brown red-brown(slightly lighter than
    sepia)

15
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16
Nap Time
17
How to anesthetize a fly
18
Viewing the Fruit Fly
19
Reference for Pictures
  • Carolina Drosophila Manual (45-2620)
  • Published by Carolina Biological Supply Company
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